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Human ES cells reveal recurrent genomic instability at 20q11.21

ABSTRACT: By analyzing five human embryonic stem (hES) cell lines over long-term culture, we detected a recurrent genomic instability in the human genome. An amplification of 2.5â4.6 Mb at 20q11.21, encompassing about 23 genes in common, appeared in four cell lines of different origins. This amplification, which has been associated with oncogenic transformation, may provide a selective advantage to hES cells in culture Array-based comparative genomic hybridization, was performed on genomic DNA samples from different human embryonic stem cell lines, all cultured in our laboratory under the same conditions. VUB05-HD is an hES cell line carrying the Huntingtin mutant gene H1, H9, SA01, and VUB01 are various hES cell lines GSM341552 and GSM341553: SNP analysis GSM341589 to GSM341669: CGH analysis

ORGANISM(S): Homo sapiens

SUBMITTER: Anselme Perrier

PROVIDER: E-GEOD-13565 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human embryonic stem cells reveal recurrent genomic instability at 20q11.21.

Lefort Nathalie N Feyeux Maxime M Bas Cécile C Féraud Olivier O Bennaceur-Griscelli Annelise A Tachdjian Gerard G Peschanski Marc M Perrier Anselme L AL

Nature biotechnology 20081123 12

By analyzing five human embryonic stem (hES) cell lines over long-term culture, we identified a recurrent genomic instability in the human genome. An amplification of 2.5-4.6 Mb at 20q11.21, encompassing approximately 23 genes in common, was detected in four cell lines of different origins. This amplification, which has been associated with oncogenic transformation, may provide a selective advantage to hES cells in culture. ...[more]

PMID: 19029913

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