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Comparison of gene expression in melanoma of wild-type and VAP-1 -/- mice

ABSTRACT: Vascular adhesion protein-1 (VAP-1) is an endothelial cell-surface protein. It is also an enzyme posessing semicarbazide-sensitive amine oxidase activity (EC.1.4.3.6). VAP-1 is involved in leukocyte traffic. To study the role of VAP-1 in tumor immunity, we compared gene expression profiles in melanomas growing in VAP-1 -/- mice and their wid-type littermates. The B16-F10-Luc-G5 melanoma cells (Xenogen) were injected subcutaneously into abdominal area of 2 wild-type and 2 VAP-1 -/- mice. Tumors were grown until day 10 when mice were euthanized. Tumors were dissected and total RNA was isolated using the Qiagen RNA extraction kit.

ORGANISM(S): Mus musculus

SUBMITTER: Fumiko Marttila-Ichihara

PROVIDER: E-GEOD-13626 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Vascular adhesion protein-1 enhances tumor growth by supporting recruitment of Gr-1+CD11b+ myeloid cells into tumors.

Marttila-Ichihara Fumiko F Auvinen Kaisa K Elima Kati K Jalkanen Sirpa S Salmi Marko M

Cancer research 20090929 19

Cancer growth is regulated by several nonmalignant cell types, such as leukocytes and endothelial cells, which reside in the stroma of the tumor. Vascular adhesion protein-1 (VAP-1) is an amine oxidase enzyme that is expressed on the surface of endothelial cells. It supports leukocyte traffic into inflamed tissues, but nothing is known about its possible role in cancer biology in vivo. Here, we report that B16 melanoma and EL-4 lymphoma remain smaller in VAP-1-deficient mice than in wild-type co ...[more]

PMID: 19789345

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