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Drosophila pseudoobscura and Drosophila simulans small RNAs

ABSTRACT: High-throughput sequencing of Drosophila pseudoobscura and Drosophila simulans small RNAs. ~18-26nt RNAs were isolated from total RNA using PAGE, ligation to adapters requires 5' monophosphate and 3' OH. Small RNAs were cloned from Drosophila pseudoobscura (heads and pooled 0-12 and 12-24 hour embryos) and Drosophila simulans (pooled 0-12 and 12-24 hour embryos). Sequencing was performed using the Illumina 1G platform. Following removal of 3' linker sequences, the clipped sequences longer than 18 nt were kept.

ORGANISM(S): Drosophila simulans

SUBMITTER: Eugene Berezikov

PROVIDER: E-GEOD-13677 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Evolutionary flux of canonical microRNAs and mirtrons in Drosophila.

Berezikov Eugene E Liu Na N Flynt Alex S AS Hodges Emily E Rooks Michelle M Hannon Gregory J GJ Lai Eric C EC

Nature genetics 20100101 1

PMID: 20037610

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