Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression profiles of HEK293 cells following induction of wild-type and mutated FOXP3

ABSTRACT: The forkhead DNA-binding protein FOXP3 is critical for the development and suppressive function of CD4+CD25+ regulatory T cells (TREG), which play a key role in maintaining self tolerance. Functionally, FOXP3 is capable of repressing transcription of cytokine genes regulated by the Nuclear Factor of Activated T cells (NFAT). Various mechanisms have been proposed by which FOXP3 mediates these effects. Using novel HEK cell lines that inducibly express either wild-type (WT) or mutant FOXP3, we have identified genome-wide expression patterns showing among other features that NFAT2 as an early target of FOXP3-mediated transcriptional repression. Six biological replicates of wild-type induced FOXP3 cell lines and six biological replicates of mutated FOXP3 cell line were studied.

ORGANISM(S): Homo sapiens

SUBMITTER: Randy Cron 

PROVIDER: E-GEOD-13798 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

FOXP3 inhibits activation-induced NFAT2 expression in T cells thereby limiting effector cytokine expression.

Torgerson Troy R TR   Genin Anna A   Chen Chunxia C   Zhang Mingce M   Zhou Bin B   Añover-Sombke Stephanie S   Frank M Barton MB   Dozmorov Igor I   Ocheltree Elizabeth E   Kulmala Petri P   Centola Michael M   Ochs Hans D HD   Wells Andrew D AD   Cron Randy Q RQ  

Journal of immunology (Baltimore, Md. : 1950) 20090629 2

The forkhead DNA-binding protein FOXP3 is critical for the development and suppressive function of CD4(+)CD25(+) regulatory T cells (T(REG)), which play a key role in maintaining self-tolerance. Functionally, FOXP3 is capable of repressing transcription of cytokine genes regulated by NFAT. Various mechanisms have been proposed by which FOXP3 mediates these effects. Using novel cell lines that inducibly express either wild-type or mutant FOXP3, we have identified NFAT2 as an early target of FOXP3  ...[more]

Similar Datasets

Proteomics Inverted chromatin immunoprecipitation identifies Tcf1 as a negative regulator of Foxp3 expression
2020-05-26 | PXD018764 | Pride
Proteomics Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells
2017-11-03 | PXD007725 | Pride
Transcriptomics Transcription profiling of mouse natural and induced regulatory T cells
2009-03-07 | E-GEOD-14415 | biostudies-arrayexpress
Proteomics Foxp3 positive and negative cell comparison
2017-02-15 | PXD001789 | Pride
Transcriptomics Transcription profiling of mouse CD4+ T cells gene-transduced with AML1, wild type Foxp3, and a Foxp3 mutant defective in binding to AML1
2008-06-15 | E-GEOD-6939 | biostudies-arrayexpress
Transcriptomics In vitro FOXP3 knockdown in human PBMCs
2024-01-08 | E-MTAB-11589 | biostudies-arrayexpress
Transcriptomics Gene expression profile of Foxp3 expressing naturally ocurring regulatory macrophages from mice
2011-04-01 | E-GEOD-23793 | biostudies-arrayexpress
Transcriptomics Regulatory and effector T cells in Lymphocytic choriomeningitis virus WE infection
2020-03-11 | E-MTAB-8861 | biostudies-arrayexpress
Transcriptomics Immune Gene Expression Profile of Conventional and Regulatory T cells
2015-04-30 | E-GEOD-61834 | biostudies-arrayexpress
Transcriptomics Conserved non-coding sequence 2 (CNS2) of the Foxp3 genomic locus is essential for the function and stability of regulatory T cells during their activation
2014-09-04 | E-GEOD-57272 | biostudies-arrayexpress