Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

The impact of BRCA2A and SSN2 on plant defense genes

ABSTRACT: BRCA2A: We used microarrays to identify differentially expressed genes. We focused on those genes that were dramatically salicylic acid-induced (>2-fold) and BRCA2A-dependent in npr1 sni1 double mutants. SSN2: We used microarrays to identify differentially expressed genes. We focused on those genes that were dramatically salicylic acid-induced (P<0.05) and SSN2-dependent in npr1 sni1 double mutants. BRCA2A: 3-week old npr1 sni1 and npr1 sni1 brca2a mutants were treated with salicylic acid for 16 hours. Replicates are derived from three independent biological experiments. SSN2: 3-week old npr1 sni1 ssn2 mutants were treated with salicylic acid for 16 hours. Replicates are derived from two (SA treated) or three (water treated) independent biological experiments.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: shui wang

PROVIDER: E-GEOD-23617 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense gene transcription during plant immune responses.

Wang Shui S Durrant Wendy E WE Song Junqi J Spivey Natalie W NW Dong Xinnian X

Proceedings of the National Academy of Sciences of the United States of America 20101213 52

Systemic acquired resistance (SAR) is a plant immune response associated with both transcriptional reprogramming and increased homologous DNA recombination (HR). SNI1 is a negative regulator of SAR and HR, as indicated by the increased basal expression of defense genes and HR in sni1. We found that the sni1 phenotypes are rescued by mutations in BREAST CANCER 2 (BRCA2). In humans, BRCA2 is a mediator of RAD51 in pairing of homologous DNA. Mutations in BRCA2 cause predisposition to breast/ovarian ...[more]

PMID: 21149701

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Project description:Zhou2015 - Circadian clock with immune regulator NPR1 Arabidopsis clock model modified from P2012 (Pokhilko et al., 2013 - BIOMD0000000445) model to include the master immune regulator NPR1 coupling to LHY, TOC1 and PRR7. Triggers: The Global Quantities contain triggers that allow one to change coupling settings, Salicyclic acid (SA) treatment and npr1 mutants. LHY_on: true->NPR1 couples to LHY PRR7_on: true->NPR1 couples to PRR7 WT: true->WT plants, false->npr1 mutant plants SA: true->SA treated plants, false->no treatment This model has L=1, i.e. operates only under constant light conditions and is not aiming to make preditions under diurnal conditions. Due to period overshoot only time points after 28h are relevant. This model is described in the article: Redox rhythm reinforces the circadian clock to gate immune response. Zhou M, Wang W, Karapetyan S, Mwimba M, Marqués J, Buchler NE, Dong X. Nature 2015 Jun; Abstract: Recent studies have shown that in addition to the transcriptional circadian clock, many organisms, including Arabidopsis, have a circadian redox rhythm driven by the organism's metabolic activities. It has been hypothesized that the redox rhythm is linked to the circadian clock, but the mechanism and the biological significance of this link have only begun to be investigated. Here we report that the master immune regulator NPR1 (non-expressor of pathogenesis-related gene 1) of Arabidopsis is a sensor of the plant's redox state and regulates transcription of core circadian clock genes even in the absence of pathogen challenge. Surprisingly, acute perturbation in the redox status triggered by the immune signal salicylic acid does not compromise the circadian clock but rather leads to its reinforcement. Mathematical modelling and subsequent experiments show that NPR1 reinforces the circadian clock without changing the period by regulating both the morning and the evening clock genes. This balanced network architecture helps plants gate their immune responses towards the morning and minimize costs on growth at night. Our study demonstrates how a sensitive redox rhythm interacts with a robust circadian clock to ensure proper responsiveness to environmental stimuli without compromising fitness of the organism. This model is hosted on BioModels Database and identified by: BIOMD0000000577. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

The impact of BRCA2A and SSN2 on plant defense genes

Publications

Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense gene transcription during plant immune responses.

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