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Oligonucleotide DNA microarray profiling identifies novel genes involved in lung adenocarcinoma


ABSTRACT: The purpose of this study was to find novel gene(s) involved in the development of lung adenocarcinomas (AD) using an oligonucleotide based DNA microarray platform with a selected set of genes from Human Apoptosis Subset v2.0 and Human Cancer Subset v3.0 (Operon). In order to identify possible novel genes involved in the development of lung ADs we analysed the expression profile of 12 lung adenocarcinomas. We used dual color approach. Tumour tissue was labeled with Cy5 and corresponding normal tissue was labeled with Cy3. Each array contained at least 4 replicate spots for each gene analysed. Gene expression was obtained by calculating median from replicate spots.

ORGANISM(S): Homo sapiens

SUBMITTER: Mojca Strazisar 

PROVIDER: E-GEOD-13947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Oligonucleotide DNA microarray profiling of lung adenocarcinoma revealed significant downregulation and deletions of vasoactive intestinal peptide receptor 1.

Mlakar Vid V   Strazisar Mojca M   Sok Mihael M   Glavac Damjan D  

Cancer investigation 20100601 5


The purpose of this study was to find novel gene(s) involved in the development of lung adenocarcinoma (AD). Using DNA microarrays, we identified 31 up-regulated and 8 downregulated genes in 12 AD. Real time PCR was used to measure expression of VIPR1 and SPP1 mRNA and possible losses or gains of genes in 32 AD. We describe significant upregulation of the SPP1 gene, downregulation of VIPR1, and losses of the VIPR1 gene. Our findings complement a proposed VIPR1 tumor suppressor role, in which del  ...[more]

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