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Differentially regulated genes induced by in vitro generated Mycobacterium tuberculosis cell wall deficient forms

ABSTRACT: Transcriptional profile of chemically generated Mycobacterium tuberculosis cell wall deficient forms, comparing untreated control cells (bacillary forms) and cell wall deficient cells. Two-condition experiment, MTB-K vs. MTB-Sph cells. Biological replicates: 3 controls, 3 spheroplasts, independently grown and harvested. In controls and spheroplasts, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, five replicates per array.

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Andrea Cossu

PROVIDER: E-GEOD-32236 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Unraveling the transcriptional regulatory networks associated to mycobacterial cell wall defective form induction by glycine and lysozyme treatment.

Rosu Valentina V Bandino Ennio E Cossu Andrea A

Microbiological research 20121126 3

It is known that a combined glycine/lysozyme treatment is able to induce in vitro the mycobacterial conversion from the bacillary to the cell wall defective forms. These forms also naturally occur in vivo as a response to various antimicrobial factors such as lysozyme released by phagocytic cells. Although they have been successfully isolated from patients with several chronic diseases, their role in pathogenesis is still unknown, mainly due to the difficulties in handling the in vivo isolated v ...[more]

PMID: 23195184

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