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Transcription profiling of human renal proximal tubular cell line HK-2 treated with Cyclosporine A timecourse 0, 12, 48 hours

ABSTRACT: Effect of 4.2 micromolar Cyclosporine A on human renal proximal tubular cell line HK-2 over 48 hours

ORGANISM(S): Homo sapiens

SUBMITTER: Craig Slattery

PROVIDER: E-GEOD-1403 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cyclosporine A-induced renal fibrosis: a role for epithelial-mesenchymal transition.

Slattery Craig C Campbell Eric E McMorrow Tara T Ryan Michael P MP

The American journal of pathology 20050801 2

Cyclosporine A, which has been the foremost immunosuppressive agent since the early 1980's, significantly improves the success of organ transplantation. However, common complications of cyclosporine A therapy, such as severe renal tubulointerstitial fibrosis, limit the drug's clinical use. Although the exact mechanisms driving cyclosporine A-induced tubulointerstitial fibrosis remain elusive, we hypothesized that epithelial-mesenchymal transition (EMT) may play a major role. We investigated this ...[more]

PMID: 16049326

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Cyclosporine A timecourse 0, 12, 48 hours

Project description:Effect of 4.2 micromolar Cyclosporine A on human renal proximal tubular cell line HK-2 over 48 hours Keywords: time-course

2004-12-20 | GSE1403 | GEO

Time-series proteomic study of diabetic nephropathy in human proximal tubular epithelial HK-2 cells

Project description:Shotgun time-series proteomic analysis based on stable isotope dimethyl labeling and UHPLC-MS/MSC to relatively quantify the changes in protein expression of human proximal tubular HK-2 cells exposed to a diabetic-like microenvironment

2021-09-08 | PXD015102 | Pride

RNAseq of HK-2 human kidney tubular cells knock-out for CNDP2

Project description:The renal proximal tubular cell line HK-2 was subjected to CRISPR-CAS9 mediated CNDP2 gene deletion.

2024-07-19 | E-MTAB-11436 | biostudies-arrayexpress

Cyclosporine on fibroblast

Project description:To elucidate the gene profile of anti-fibroproliferative effects of cyclosporine, we added TGF-b and/or CsA to MRC5 (fetal human lung fibroblast) cell line. After 24 hours serum starvation, fibroblasts were treated with 3 ng/ml TGF-β1 and were treated with or without 2μg/ml Cyclosporine and effects were examined at 48 hours after treatment. Expression of three genes (IGFBP3, ID1 and PPARG) from this signature was quantified in the same RNA samples by real-time PCR. TGF-β and cyclosporine induced gene expression data was measured in MRC5 (fetal human fibroblast cell line) at 48h after treatments. The dose of TGF-β was 3 ng/ml and cyclosporine was 2 μg/ml. The comparison was done between four groups (control, TGF-β, cyclosporine and TGF-β plus cyclosporine).Three independent experiments were performed at each treatments using different samples for each experiment.

2010-11-09 | E-GEOD-25200 | biostudies-arrayexpress

Transcription profiling of human mono or co-cultured HMEC-1 and HK-2 cells to identify the influence of microvascular endothelial cells on transcriptional regulation of proximal tubular epithelial cells

Project description:The effect of the presence of HMEC-1 cells on HK-2 cell gene expression was investigated. Cells were cultured in mono or co-culture on opposite sides of aluminium oxide filters, 0.2 ?µM pore size. These conditions provide a close proximity but not direct contact. RNA from HK-2 cells was assayed with Affymetrix HGU133 Plus 2 arrays. 3 Biological samples were used per group.

2008-02-05 | E-MEXP-1178 | biostudies-arrayexpress

Effect of the sGC activator BAY 60-2770 on human proximal tubular (HK-2) cells under hypoxia and after TGF-β1 treatment

Project description:Increasing cGMP levels by modulating soluble guanylyl cyclase (sGC) can be renoprotective. While sGC stimulators can modulate cGMP levels, their application is limited as they require native sGC to function. sGC activators on the other hand can function with heme-free sGC. Therefore, we tested the renoprotective effects of the sGC activator BAY 60-2770 that also functions with oxidized sGC in an acute kidney injury (AKI) model with transition to chronic kidney disease (CKD). In order to study the effects of BAY 60-2770 on gene expression in tubular cells, we used the proximal tubular cell line HK-2 as a model. To mimic the ischemia and fibrosis observed in vivo, we subjected HK-2 cells to hypoxia and TGF-β1 treatment, respectively. RNASeq analysis showed that BAY 60-2770 had a significant influence on the expression of genes involved in fibrosis, inflammation and tissue damage after both hypoxia and TGF-β1 treatments.

2024-11-05 | GSE269418 | GEO

Cyclosporine on fibroblast

2010-11-09 | GSE25200 | GEO

Saccharospirillum mangrovi strain:HK-33 | isolate:HK-33

Project description:Saccharospirillum mangrovi strain:HK-33 | isolate:HK-33 Genome sequencing

| PRJNA483515 | ENA

Candidatus Magnetomorum sp. HK-1 isolate:HK-1

Project description:Candidatus Magnetomorum sp. HK-1 isolate:HK-1 Genome sequencing and assembly

| PRJNA252699 | ENA

Detection Novel miRNA/mRNA Potentially Involved in Renal Proximal Tubular Epithelial Cells under DM Using Next-Generation Sequencing and Bioinformatic Approaches

Project description:MicroRNAs/mRNAs are hypothesized to play critical roles in the regulation of DM-induced proximal tubular injury. The aim of this study is to explore novel microRNAs differentially expressed in HK-2 cells under normoxia and hypoxia conditions. RNAs were extracted from HK-2 cells cultured under normoxia and hypoxia for sequencing. Using the next generation sequencing and bioinformatics approaches, we identified 11 differentially expressed microRNAs in HK-2 cells under hypoxia condition.

2022-01-10 | GSE185586 | GEO

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