Dataset Information

Time-series proteomic study of diabetic nephropathy in human proximal tubular epithelial HK-2 cells

ABSTRACT: Shotgun time-series proteomic analysis based on stable isotope dimethyl labeling and UHPLC-MS/MSC to relatively quantify the changes in protein expression of human proximal tubular HK-2 cells exposed to a diabetic-like microenvironment

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Alberto Valdés

LAB HEAD: María Luisa Marina

PROVIDER: PXD015102 | Pride | 2021-09-08

REPOSITORIES: Pride

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Time-series proteomic study of the response of HK-2 cells to hyperglycemic, hypoxic diabetic-like milieu.

Valdés Alberto A Castro-Puyana María M García-Pastor Coral C Lucio-Cazaña Francisco J FJ Marina María Luisa ML

PloS one 20200624 6

During diabetes, renal proximal tubular cells (PTC) are exposed to a combination of high glucose and hypoxic conditions, which plays a relevant role in the development of diabetic kidney disease (DKD). In this work, a time-series proteomic study was performed to analyse the effect of a diabetic-like microenvironment induced changes on HK-2 cells, a human cell line derived from normal proximal tubular epithelial cells. Cells simultaneously exposed to high glucose (25 mM) and hypoxia (1% O2) were ...[more]

PMID: 32579601

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