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Gene category-specific regulation of transcription elongation across the yeast genome

ABSTRACT: Analysis of genome-wide differences of transcription using Genomic run-on (GRO), RNApol II ChIP-on-Chip, cDNA analysis and ChIP-on-Chip. This SuperSeries is composed of the following subset Series: GSE14060 RNA pol II ChIP on chip (RPCC) GSE14077 RPCC analysis of rap1-sil, tpk1 & tpk2 GSE14080 GRO analysis of rap1-sil, tpk1 & tpk2 GSE14082 Analysis of Spt16 depletion GSE1002 YPD to YPGal timecourse Refer to individual Series

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Alfonso Rodriguez-Gil

PROVIDER: E-GEOD-14084 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulon-specific control of transcription elongation across the yeast genome.

Pelechano Vicent V Jimeno-González Silvia S Rodríguez-Gil Alfonso A García-Martínez José J Pérez-Ortín José E JE Chávez Sebastián S

PLoS genetics 20090821 8

Transcription elongation by RNA polymerase II was often considered an invariant non-regulated process. However, genome-wide studies have shown that transcriptional pausing during elongation is a frequent phenomenon in tightly-regulated metazoan genes. Using a combination of ChIP-on-chip and genomic run-on approaches, we found that the proportion of transcriptionally active RNA polymerase II (active versus total) present throughout the yeast genome is characteristic of some functional gene classe ...[more]

PMID: 19696888

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