Unknown,Transcriptomics,Genomics,Proteomics

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Transcription rate analysis of W303 hog1 mutant strain subjected to osmotic stress


ABSTRACT: Transcription rate (TR) analysis of W303 hog1 mutant yeast strain growing in exponential phase in YPD subjected to osmotic stress Keywords: Time course Transcription rate analysis by means of GRO of three independent replicates the yeast strain growing in exponential phase. Each time point replicate has been hybridized on a different macroarray (F18-F24).

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jose Perez-Ortin 

PROVIDER: E-GEOD-13098 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Specific and global regulation of mRNA stability during osmotic stress in Saccharomyces cerevisiae.

Romero-Santacreu Lorena L   Moreno Joaquín J   Pérez-Ortín José E JE   Alepuz Paula P  

RNA (New York, N.Y.) 20090415 6


Hyperosmotic stress yields reprogramming of gene expression in Saccharomyces cerevisiae cells. Most of this response is orchestrated by Hog1, a stress-activated, mitogen-activated protein kinase (MAPK) homologous to human p38. We investigated, on a genomic scale, the contribution of changes in transcription rates and mRNA stabilities to the modulation of mRNA amounts during the response to osmotic stress in wild-type and hog1 mutant cells. Mild osmotic shock induces a broad mRNA destabilization;  ...[more]

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