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Expression profiling of PaTu8988s and PaTu8988t cell lines

ABSTRACT: We used microarray to look at the genes deregulated in PaTu8988s (adenovirus insensitive) and PaTu8988t (adenovirus sensitive) cell lines PaTu8988s and PaTu8988t are pancreatic cell lines derived from the same patient and show significant responses to oncolytic adenoviruses. The different response to adenovirus in these cell lines is not related to the previously known mechanisms that affect adenovirus infection. The two cell lines represent represent a "suitable" model to screen tumour-associated genes affecting the potency of aoncolytic adenovirus. RNA was extracted and hybridized on Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: yaohe Wang

PROVIDER: E-GEOD-14701 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CEACAM6 attenuates adenovirus infection by antagonizing viral trafficking in cancer cells.

Wang Yaohe Y Gangeswaran Rathi R Zhao Xingbo X Wang Pengju P Tysome James J Bhakta Vipul V Yuan Ming M Chikkanna-Gowda C P CP Jiang Guozhong G Gao Dongling D Cao Fengyu F Francis Jennelle J Yu Jinxia J Liu Kangdong K Yang Hongyan H Zhang Yunhan Y Zang Weidong W Chelala Claude C Dong Ziming Z Lemoine Nick N

The Journal of clinical investigation 20090601 6

The changes in cancer cell surface molecules and intracellular signaling pathways during tumorigenesis make delivery of adenovirus-based cancer therapies inefficient. Here we have identified carcinoembryonic antigen- related cell adhesion molecule 6 (CEACAM6) as a cellular protein that restricts the ability of adenoviral vectors to infect cancer cells. We have demonstrated that CEACAM6 can antagonize the Src signaling pathway, downregulate cancer cell cytoskeleton proteins, and block adenovirus ...[more]

PMID: 19411761

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Expression profiling of PaTu8988s and PaTu8988t cell lines

Project description:We used microarray to look at the genes deregulated in PaTu8988s (adenovirus insensitive) and PaTu8988t (adenovirus sensitive) cell lines PaTu8988s and PaTu8988t are pancreatic cell lines derived from the same patient and show significant responses to oncolytic adenoviruses. The different response to adenovirus in these cell lines is not related to the previously known mechanisms that affect adenovirus infection. The two cell lines represent represent a "suitable" model to screen tumour-associated genes affecting the potency of aoncolytic adenovirus.

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