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Gene expression profiles from osteosarcpma samples

ABSTRACT: Osteosarcoma patients with development of pulmonary metastasis have still poorer prognosis in spite of aggressive treatment. However, molecular mechanism of metastasis is still unknown. We analysed the expression levels of 54,613 probe sets in surgical 27 samples of osteosarcoma with GeneChip Human Genome U133 Plus 2.0 arrays. Keywords: gene expression array-based, count Total RNA was extracted from 27 fresh frozen tumour specimens. We analysed the global gene exprssion profiles of these osteosarcoma cases in order to clarify the genomic basis behind the development of pulmonary metastasis.

ORGANISM(S): Homo sapiens

DISEASE(S): osteosarcoma subtype fibroblastic

SUBMITTER: Reiko Satow

PROVIDER: E-GEOD-14827 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reduced argininosuccinate synthetase is a predictive biomarker for the development of pulmonary metastasis in patients with osteosarcoma.

Kobayashi Eisuke E Masuda Mari M Nakayama Robert R Ichikawa Hitoshi H Satow Reiko R Shitashige Miki M Honda Kazufumi K Yamaguchi Umio U Shoji Ayako A Tochigi Naobumi N Morioka Hideo H Toyama Yoshiaki Y Hirohashi Setsuo S Kawai Akira A Yamada Tesshi T

Molecular cancer therapeutics 20100216 3

Pulmonary metastasis is the most significant prognostic determinant for osteosarcoma, but methods for its prediction and treatment have not been established. Using oligonucleotide microarrays, we compared the global gene expression of biopsy samples between seven osteosarcoma patients who developed pulmonary metastasis within 4 years after neoadjuvant chemotherapy and curative resection, and 12 patients who did not relapse. We identified argininosuccinate synthetase (ASS) as a gene differentiall ...[more]

PMID: 20159990

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