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Expression profile of ZOS and ZOSM osteosarcoma cell lines

ABSTRACT: Pulmonary metastasis is the main cause of medical failure and death of osteosarcoma patients. Despite intensive search for new therapeutic strategies, survival has not improved during the last two decades. Therefore, it’s very urgent to understand the underlying mechanisms of tumor progression to identify targets of novel therapies for osteosarcoma. We used microarrays to identify the metastasis-driving gene during osteosarcoma metastasis Microarrays are performed in ZOS and ZOSM-two syngenic primary human osteosarcoma cell lines with low and high metastatic potential which are established in our lab

ORGANISM(S): Homo sapiens

SUBMITTER: Jinchang Lu

PROVIDER: E-GEOD-55957 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IRX1 hypomethylation promotes osteosarcoma metastasis via induction of CXCL14/NF-κB signaling.

Lu Jinchang J Song Guohui G Tang Qinglian Q Zou Changye C Han Feng F Zhao Zhiqiang Z Yong Bicheng B Yin Junqiang J Xu Huaiyuan H Xie Xianbiao X Kang Tiebang T Lam YingLee Y Yang Huiling H Shen Jingnan J Wang Jin J

The Journal of clinical investigation 20150330 5

Osteosarcoma is a common malignant bone tumor with a propensity to metastasize to the lungs. Epigenetic abnormalities have been demonstrated to underlie osteosarcoma development; however, the epigenetic mechanisms that are involved in metastasis are not yet clear. Here, we analyzed 2 syngeneic primary human osteosarcoma cell lines that exhibit disparate metastatic potential for differences in epigenetic modifications and expression. Using methylated DNA immunoprecipitation (MeDIP) and microarray ...[more]

PMID: 25822025

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Expression profile of ZOS and ZOSM osteosarcoma cell lines

Project description:Pulmonary metastasis is the main cause of medical failure and death of osteosarcoma patients. Despite intensive search for new therapeutic strategies, survival has not improved during the last two decades. Therefore, it’s very urgent to understand the underlying mechanisms of tumor progression to identify targets of novel therapies for osteosarcoma. We used microarrays to identify the metastasis-driving gene during osteosarcoma metastasis

2015-08-25 | GSE55957 | GEO

Expression profiling of 143B-shCtrl and 143B-shIRX1 stable osteosarcoma cell lines

Project description:Pulmonary metastasis is the main cause of medical failure and death of osteosarcoma patients. Our recent study identified IRX1 as a potential metastasis-driving gene in osteosarcoma. Studies showed that IRX1 can promote the migration, invasion and anoikis resistance of osteosarcoma cells. We generated 143B stable IRX1 knockdown and control cell lines, and found that IRX1 knockdown can inhibit the pulmonary metastasis of 143B cells in orthotopic mouse osteosarcoma model. Expression microarrays are performed in143B-shCtrl and 143B-shIRX1 cells to study the mechanism of IRX1 on promoting metastasis of osteosarcoma

2015-08-25 | E-GEOD-55958 | biostudies-arrayexpress

Epigenetic screen of metastasis-driving genes in osteosarcoma

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-08-25 | E-GEOD-56255 | biostudies-arrayexpress

Expression analysis of LM8 osteosarcoma cells overexpressing human Prolyl Hydroxylase Domain Protein-4 (PHD4) and LM8 control cells

Project description:PHD4 regulates the expression of Hypxia-inducible Factor 2 (HIF-2) alpha in LM8 osteosarcoma cells. PHD4 overexpression inhibits the growth of experimental tumor in syngenic mice but stimulates angiogenesis via Transforming Growth-Factor (TGF)-alpha. We used microarrays to investigate the gene expression of stable LM8 cell line overexpressing human PHD4 in comparison to the LM8 control line, transfected with the empty vector. RNA was prepared from cultured cells and used for hybridization on Affymetrix microarrays.

2013-08-09 | E-GEOD-49680 | biostudies-arrayexpress

MeDIP chip from ZOS and ZOSM osteosarcoma cell lines

Project description:Epigenetic change of genes expression, including hypomethylation-linked activation of oncogenes and hypermethylation-associated inactivation of tumor suppressor genes, can affect almost all the cellular signaling pathways that participate in cancer initiation and progression. Unlike genetic alterations, epigenetic changes are potentially reversible, making them attractive and promising targets for therapeutic intervention. Studies indicated that abnormal DNA methylation is involved in dysregulation of cell cycle, apoptosis, proliferation and differentiation of osteosarcoma. However, epigenetic mechanisms of osteosarcoma metastasis remain largely unknown. MeDIP chips are performed in ZOS and ZOSM—two syngeneic primary human osteosarcoma cell lines with low and high metastatic potential which are established in our lab

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Gene expression profiles from osteosarcpma samples

Project description:Osteosarcoma patients with development of pulmonary metastasis have still poorer prognosis in spite of aggressive treatment. However, molecular mechanism of metastasis is still unknown. We analysed the expression levels of 54,613 probe sets in surgical 27 samples of osteosarcoma with GeneChip Human Genome U133 Plus 2.0 arrays. Keywords: gene expression array-based, count Total RNA was extracted from 27 fresh frozen tumour specimens. We analysed the global gene exprssion profiles of these osteosarcoma cases in order to clarify the genomic basis behind the development of pulmonary metastasis.

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Expression analysis of LM8 osteosarcoma cells overexpressing human Prolyl Hydroxylase Domain Protein-4 (PHD4) and LM8 control cells

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Project description:Aberrations in the Hedgehog (Hh) pathway are known to related to several malignancies. However, little is known about the function of GLI2, a transcription factor in the Hh pathway, in osteosarcoma. Osteosarcoma is the most frequent primary bone sarcoma in children and adolescents. Despite survival rates of osteosarcoma patients have increased, the prognosis of patients with metastasis remains poor. Therefore, the development of novel therapeutic strategies for osteosarcoma patients is development of novel therapeutic strategies for osteosarcoma patients is urgently needed. Aberrations in the Hedgehog (Hh) pathway are known to related to several malignancies. However, little is known about the function of GLI2, a transcription factor in the Hh pathway, in osteosarcoma. Our findings revealed that GLI2 was overexpressed in osteosarcoma tissues. Additionally, GLI2 is involved in the metastasis of osteosarcoma cells through the regulation of ribosomal protein S3 expression. Furthermore, we showed that arsenic trioxide (ATO) suppressed the invasion and lung metastasis of osteosarcoma cells by the inhibition of GLI transcription. Consequently, these finding reveal a novel function of GLI2 in the metastasis of osteosarcoma and that ATO may be a new therapeutic agentay be a new therapeutic agent. We revealed that a novel function of GLI2 in the metastasis of osteosarcoma and that ATO may be a new therapeutic agent for preventing osteosarcoma metastasis. Negative siRNA(U-2OS) and GLI2 siRNA(U-2OS)

2012-12-14 | E-GEOD-42903 | biostudies-arrayexpress

Expression profiling of 143B-shCtrl and 143B-shIRX1 stable osteosarcoma cell lines

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Gene expression profiles of human osteosarcoma, set2

Project description:Pulmonary metastasis continues to be the most common cause of death in osteosarcoma. Indeed, the 5-year survival for newly diagnosed osteosarcoma patients has not significantly changed in over 20 years. Further understanding of the mechanisms of metastasis and resistance for this aggressive pediatric cancer is necessary. Pet dogs naturally develop osteosarcoma providing a novel opportunity to model metastasis development and progression. Given the accelerated biology of canine osteosarcoma, we hypothesized that a direct comparison of canine and pediatric osteosarcoma expression profiles may help identify novel metastasis-associated tumor targets that have been missed through the study of the human cancer alone. Collectively, these data support the strong similarities between human and canine osteosarcoma and underline the opportunities provided by a comparative oncology approach as a means to improve our understanding of cancer biology and therapy. Profiles of human osteosarcoma, single channel design

2009-11-04 | E-GEOD-16091 | biostudies-arrayexpress

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