Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis Seedlings treated with Salicylic Acid

ABSTRACT: Disease resistance is associated with a plant defense response that involves an integrated set of signal transduction pathways. Gene Expression studies were performed using Arabidopsis wild type seedlings treated with and without salicylic acid (SA). In this study we examined pathogenesis related genes are upregulated. We used Affymetrix GeneChip to study the genome wide gene expression with and without induction of defense by treatment with SA Experiment Overall Design: Arabidopsis seedlings Col-0 were grown for 9 days in controlled environment rooms at 22Â°C temperature and a photoperiod of 16 h light 100 Âµm m2 s_1 cool-white fluorescent illumination. After 9 days seedlings were transferred into water as a mock treatment and with the defense related signaling molecules salicylic acid (SA) for one day. The concentration of SA (Sigma, St. Louis) was 2mM and pH 6.5. RNA extracted from SA and mock treated seedling and hybridize with Affymetrix GeneChip to examine the change in plant defence gene expression due to induction of SA.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Samir Sawant

PROVIDER: E-GEOD-14961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:As sessile organism, plants evolved a highly complicated signaling system to cope with unfavorable and fluctuating environmental conditions. Rapid and transient Reactive Oxygen Species (ROS) burst is a common response to both biotic and abiotic stresses. Plants exposed with O3 could trigger extracellular similar ROS production through cell wall peroxidases and NPADPH oxidases, resulting in changes in the gene expression and cell death. Whereas ROS induced cell death is not simply due to its toxicity, rather due to interplay with several other signaling pathways, such as salicylic acid (SA), jasmonic acid (JA) and ethylene signaling pathways. Furthermore, the three hormones have both synergistic and antagonistic interactions, where the suppression of JA signaling by SA is the mostly studied. In addition, ethylene promotes cell death while JA has a protective role upon O3 exposure. The role of SA is more complicated; depending on the genetic background it can have either cell death promoting or protecting roles. Hence, a clean system to deliver apoplastic ROS is required to study the role of ROS apart from con-current activation of other signaling pathways. Arabidopsis thaliana offer a convenient system to study apoplastic ROS signaling due to the availability of hormone signaling or biosynthesis mutants including the JA receptor mutant coi1-16 (CORONATINE INSENSITIVE1), the essential ethylene signaling mutant ein2 (ETHYLENE INSENSITIVE2), the SA biosynthesis mutant sid2 (SALICYLIC ACID INDUCTION DEFICIENT2 also known as ISOCHORISMATE SYNTHASE1), and essential regulators in SA/JA/ethylene-induced defense response triple mutant tga2 tga5 tga6 (Clade II TGA transcription factors). Here we used a combination of transcriptome analysis, cell death assays and mutant analysis to systematically quantified the contribution of hormone signaling in relation to apoplastic ROS signaling, identified transcription factors (TFs) involved in ROS regulation and dissected the components involved in defense hormones associated cell death. Transcriptome profiling of ozone response using two arabidopsis triple mutants coi1-16 ein2 sid2 and tga2 tga5 tga6 related to Jasmonic acid, salicylic acid and ethylene signaling to identify hormone-independant apoplastic ROS signaling

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Transcription profiling of Arabidopsis Seedlings treated with Salicylic Acid

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