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Expression data from Arabidopsis Seedlings treated with Salicylic Acid

ABSTRACT: Disease resistance is associated with a plant defense response that involves an integrated set of signal transduction pathways. Gene Expression studies were performed using Arabidopsis wild type seedlings treated with and without salicylic acid (SA). In this study we examined pathogenesis related genes are upregulated. We used Affymetrix GeneChip to study the genome wide gene expression with and without induction of defense by treatment with SA

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE14961 | GEO | 2009/02/24

SECONDARY ACCESSION(S): PRJNA111835

REPOSITORIES: GEO

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Transcription profiling of Arabidopsis Seedlings treated with Salicylic Acid

Project description:Disease resistance is associated with a plant defense response that involves an integrated set of signal transduction pathways. Gene Expression studies were performed using Arabidopsis wild type seedlings treated with and without salicylic acid (SA). In this study we examined pathogenesis related genes are upregulated. We used Affymetrix GeneChip to study the genome wide gene expression with and without induction of defense by treatment with SA Experiment Overall Design: Arabidopsis seedlings Col-0 were grown for 9 days in controlled environment rooms at 22Â°C temperature and a photoperiod of 16 h light 100 Âµm m2 s_1 cool-white fluorescent illumination. After 9 days seedlings were transferred into water as a mock treatment and with the defense related signaling molecules salicylic acid (SA) for one day. The concentration of SA (Sigma, St. Louis) was 2mM and pH 6.5. RNA extracted from SA and mock treated seedling and hybridize with Affymetrix GeneChip to examine the change in plant defence gene expression due to induction of SA.

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