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Comparative genome hybridization for Lactobacillus casei

ABSTRACT: Lactobacillus casei is remarkably adaptive to diverse habitats. To understand the evolution and adaptation of Lb. casei strains isolated from different environments, the gene content of 22 Lb. casei strains isolated from various habitats (cheeses, n=8; plant materials, n=8; and human sources, n=6) were examined by comparative genome hybridization with an Lb. casei ATCC 334-based microarray. Comparative genome hybridization was performed against an Affymetrix custom microarray designed to include 2,661 (97%) chromosomal and 17 (85%) plasmid CDSs predicted to occur in Lb. casei ATCC 334, as well as all predicted CDSs in the draft Lb. helveticus CNRZ 32 genome. CDSs that were not included in the microarray design were all transposase-encoding genes.

ORGANISM(S): Lactobacillus casei

SUBMITTER: James Steele

PROVIDER: E-GEOD-15030 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome sequence and comparative genome analysis of Lactobacillus casei: insights into their niche-associated evolution.

Cai Hui H Thompson Rebecca R Budinich Mateo F MF Broadbent Jeff R JR Steele James L JL

Genome biology and evolution 20090714

Lactobacillus casei is remarkably adaptable to diverse habitats and widely used in the food industry. To reveal the genomic features that contribute to its broad ecological adaptability and examine the evolution of the species, the genome sequence of L. casei ATCC 334 is analyzed and compared with other sequenced lactobacilli. This analysis reveals that ATCC 334 contains a high number of coding sequences involved in carbohydrate utilization and transcriptional regulation, reflecting its requirem ...[more]

PMID: 20333194

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