Project description:To identify biological differences between primitive and monocytic primary AML, we sorted ROS-low enriched LSC subfractions from phenotypically monocytic (CD45-bright/SSC-high/CD117-/CD11b+/CD68+/CD64+) and primitive AML (CD45-medium/SSC-low/CD117+/CD11b-/CD68-/CD64-) specimens and performed RNA-seq analysis.
Project description:Gene-expression changes in U937 cells undergoing monocytic differentiation with TPA treatment Keywords: differentiation treatment The promonocytic U937 cell line was maintained at 37C in RPMI 1640 medium (Mediatech) supplemented with 10% fetalclone (HyClone) under a 5% CO2, 95% air atmosphere. U937 cells were differentiated by the addition to the growth medium 100 nM 12-O-tetradeconoyl-phorbol 13-acetate (TPA) (Sigma).Three condition experiment, treated with TPA for two different time points (27 and 96 hrs) vs. untreated. Biological replicates: independently grown, treated and harvested
Project description:Using RNA-seq, we report here that anthrax lethal toxin (LeTx) induces immunosuppression in human lung epithelial cell line A549 and monocytic cell line U937.
Project description:Since the expression profile of miRNAs is specific in different tissues or under different physiological conditions, the correlations between miRNAs and mRNAs could vary under different biological circumstances. This is a study also used expression profiles of miRNA and mRNA during monocytic differentiation to explore the correlations between the expression levels of miRNAs and mRNA, either negative or positive. After treatment of TPA, U937 cells present functional differentiation markers as well as specific cell morphology, which were confirmed by flow cytometric analysis and Wright-Giemsa staining.Here we use genome-wide microarray analysis to profile the expression levels of mRNA in normal U937 cells and differentiated U937 cells. For each differentiated and control sample, two hybridizations were performed by using a reversal fluorescent strategy.
Project description:Pro-monocytic U937 cells were subjected to FURIN gene editing via an optimized CRISPR technology, resulting in clones with 1 or both copies of FURIN deleted. Paired-end RNA sequencing was performed on these cells, in addition to wild-type cells, to determine the effects of FURIN depletion on the transcriptome.
Project description:Since the expression profile of miRNAs is specific in different tissues or under different physiological conditions, the correlations between miRNAs and mRNAs could vary under different biological circumstances. This is a study also used expression profiles of miRNA and mRNA during monocytic differentiation to explore the correlations between the expression levels of miRNAs and mRNA, either negative or positive. After treatment of TPA, U937 cells present functional differentiation markers as well as specific cell morphology, which were confirmed by flow cytometric analysis and Wright-Giemsa staining.Here we use a miRNA microarray (CapitalBio, Beijing, China) containing 509 well-characterized human, mouse and rat miRNAs and various controls to profile the expression levels of miRNA in normal U937 cells and differentiated U937 cells. Three independent biological experiments were performed, and for each differentiated and control sample, two hybridizations were performed by using a reversal fluorescent strategy.
Project description:The human leukemia cell line U937 is a well-established model for studying monocytic cell differentiation. We used a modified protocol (SADE) of serial analysis of gene expression (SAGE) and developed a SADE linker-anchored PCR assay to investigate the pattern of expression of known genes and to identify new transcripts in proliferating cells and during cell growth arrest and differentiation. We implemented new informatic tools to compare expression profiles before and after exposure of cells to differentiation inducers. From the analysis of 47,388 tags, we identified 13,806 distinct transcripts, 265 of which showed significant variations (P<0.01). Among 1219 well-identified genes, major changes concerned transcription and translation components, cytoskeleton, and macrophage-specific genes. Nearly half of the tags, some of them expressed at high levels, matched partially characterized genes or ESTs, or revealed yet-unknown transcripts, providing a wealth of new candidate genes that may reveal novel aspects of terminal monocytic differentiation. Keywords = 12213207 Keywords: other