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Depletion of Erk1 and Erk2 MAP kinases in primary human keratinocytes

ABSTRACT: We assessed the effect of RNAi-mediated MAP kinase cascade signaling blockade in primary human keratinocytes. Two sets of siRNA targeting different regions of the Erk1/2 genes were used, enabling identification of off-target siRNA effects. Primary human keratinocytes were electroporated with scrambled control siRNA, a pair of siRNA oligomers against Erk1 and Erk2 (set A), or an independent pair of siRNA oligomers against Erk1 and Erk2 (set B). Four days after electroporation, RNA was harvested. Three biological replicates were performed for each of the three siRNA groups.

ORGANISM(S): Homo sapiens

SUBMITTER: Phillip Dumesic

PROVIDER: E-GEOD-15417 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Erk1/2 MAP kinases are required for epidermal G2/M progression.

Dumesic Phillip A PA Scholl Florence A FA Barragan Deborah I DI Khavari Paul A PA

The Journal of cell biology 20090501 3

Erk1/2 mitogen-activated protein kinases (MAPKs) are often hyperactivated in human cancers, where they affect multiple processes, including proliferation. However, the effects of Erk1/2 loss in normal epithelial tissue, the setting of most extracellular signal-regulated kinase (Erk)-associated neoplasms, are unknown. In epidermis, loss of Erk1 or Erk2 individually has no effect, whereas simultaneous Erk1/2 depletion inhibits cell division, demonstrating that these MAPKs are necessary for normal ...[more]

PMID: 19414607

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Depletion of Erk1 and Erk2 MAP kinases in primary human keratinocytes

Project description:We assessed the effect of RNAi-mediated MAP kinase cascade signaling blockade in primary human keratinocytes. Two sets of siRNA targeting different regions of the Erk1/2 genes were used, enabling identification of off-target siRNA effects.

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