Unknown,Transcriptomics,Genomics,Proteomics

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SiRNA of human MCF-7 cells


ABSTRACT: Overexpression of the AP-2γ transcription factor in breast tumours has been identified as an independent predictor of poor outcome and failure of hormone therapy, even in ER positive, ErbB2 negative tumours; markers of a more favourable prognosis. To understand further the role of AP-2γ in breast carcinoma, we have used an RNA interference and gene expression profiling strategy using the MCF-7 cell line as a model for ER positive, ErbB2 negative tumours with AP-2γ overexpression. Gene expression changes between control and silenced cells implicate AP-2γ in the control of cell cycle progression and developmental signalling. Experiment Overall Design: We compared the expression profiles of MCF-7 cells separately transfected with three independent AP-2γ targeting sequences with those from control cells treated with transfection reagent alone or a non-silencing control siRNA on Affymetrix arrays; each condition was examined in triplicate.

ORGANISM(S): Homo sapiens

SUBMITTER: Helen Hurst 

PROVIDER: E-GEOD-15481 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

AP-2gamma promotes proliferation in breast tumour cells by direct repression of the CDKN1A gene.

Williams Christopher M J CM   Scibetta Angelo G AG   Friedrich J Karsten JK   Canosa Monica M   Berlato Chiara C   Moss Charlotte H CH   Hurst Helen C HC  

The EMBO journal 20091001 22


Overexpression of the activator protein (AP)-2gamma transcription factor in breast tumours has been identified as an independent predictor of poor outcome and failure of hormone therapy. To understand further the function of AP-2gamma in breast carcinoma, we have used an RNA interference and gene expression profiling strategy with the MCF-7 cell line as a model. Gene expression changes between control and silenced cells implicate AP-2gamma in the control of cell cycle progression and development  ...[more]

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