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Expression data from batch cultivations with A. niger, A. oryzae and A. nidulans grown on glucose and glycerol

ABSTRACT: Conserved transcriptional regulation of glycerol metabolism was investigated in three Aspergillus species, A. nidulans, A. oryzae and A. niger. For this purpose, batch cultivations under well controlled conditions were performed with the three Aspergilli. Samples for RNA extraction were collected and further processed for hybridization in custom designed Affymetrix microarrays containing probes for the three Aspergillus species. Protein comparisons and cross analysis with gene expression data of all three species resulted in the identification of 88 genes having a conserved response across the three Aspergillus species. A promoter analysis of the up-regulated genes led to the identification of a conserved binding site for a putative regulator. Triplicate batch fermentations with each of the three Aspergillus species were carried out and transcriptome analysis was performed. Biomass from each Aspergillus species was harvested in the exponential phase of growth and further processed for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Aspergillus oryzae

SUBMITTER: Wanwipa Vongsangnak 

PROVIDER: E-GEOD-15702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Uncovering transcriptional regulation of glycerol metabolism in Aspergilli through genome-wide gene expression data analysis.

Salazar Margarita M   Vongsangnak Wanwipa W   Panagiotou Gianni G   Andersen Mikael R MR   Nielsen Jens J  

Molecular genetics and genomics : MGG 20090926 6

Glycerol is catabolized by a wide range of microorganisms including Aspergillus species. To identify the transcriptional regulation of glycerol metabolism in Aspergillus, we analyzed data from triplicate batch fermentations of three different Aspergilli (Aspergillus nidulans, Aspergillus oryzae and Aspergillus niger) with glucose and glycerol as carbon sources. Protein comparisons and cross-analysis with gene expression data of all three species resulted in the identification of 88 genes having  ...[more]

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