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Mixed colonies of Aspergillus niger and Aspergillus oryzae cooperatively degrading wheat bran

ABSTRACT: A. niger and A. oryzae are two filamentous fungi widely used in industry to produce various enzymes (e.g. pectinases, amylases) and metabolites (e.g. citric acid). Using proteomics, the co-cultivation of these two fungi in wheat bran showed an equal distribution of the two strains forming mixed colonies with a broad range of carbohydrate active enzymes produced. This stable mixed microbial system seems suitable for subsequent commercial processes such as enzyme production. XlnR knock-out strains for both aspergilli were used to study the influence of plant cell wall degrading enzyme production on the fitness of the mixed culture.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Aspergillus Oryzae Var. Oryzae Aspergillus Niger

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD005532 | Pride | 2017-02-27

REPOSITORIES: Pride

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			Action	DRS
	IB10_AoryzaeFP101_5_Low.RAW	Raw
	IB11_AnigerCB119_1_High.RAW	Raw
	IB12_AnigerCB119_2.RAW	Raw
	IB13_AnigerCB119_3.RAW	Raw
	IB14_AnigerCB119_4.RAW	Raw

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Mixed colonies of Aspergillus niger and Aspergillus oryzae cooperatively degrading wheat bran.

Benoit-Gelber I I Gruntjes T T Vinck A A van Veluw J G JG Wösten H A B HAB Boeren S S Vervoort J J M JJM de Vries R P RP

Fungal genetics and biology : FG & B 20170220

In both natural and man-made environments, microorganisms live in mixed populations, while in laboratory conditions monocultures are mainly used. Microbial interactions are often described as antagonistic, but can also be neutral or cooperative, and are generally associated with a metabolic change of each partner and cause a change in the pattern of produced bioactive molecules. A. niger and A. oryzae are two filamentous fungi widely used in industry to produce various enzymes (e.g. pectinases, ...[more]

PMID: 28232095

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Project description:The transcriptional activator XlnR (Xlr1/Xyr1) is a major regulator in fungal xylan and cellulose degradation as well as in the utilization of d-xylose via the pentose catabolic pathway. XlnR homologs are commonly found in filamentous ascomycetes and often assumed to have the same function in different fungi. However, a comparison of the saprobe Aspergillus niger and the plant pathogen Magnaporthe oryzae showed different phenotypes for deletion strains of XlnR. In this study wild type and xlnR/xlr1/xyr1 mutants of five fungi were compared: Fusarium graminearum, M. oryzae, Trichoderma reesei, A. niger and Aspergillus nidulans. Growth profiling on relevant substrates and a detailed analysis of the secretome as well as extracellular enzyme activities demonstrated a common role of this regulator in activating genes encoding the main xylanolytic enzymes. However, large differences were found in the set of genes that is controlled by XlnR in the different species, resulting in the production of different extracellular enzyme spectra by these fungi. This comparison emphasizes the functional diversity of a fine-tuned (hemi-)cellulolytic regulatory system in filamentous fungi, which might be related to the adaptation of fungi to their specific biotopes.

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