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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human embryonic kidney cells HEK293 overexpressing the transcription factors HNF4a2 or mutant forms HNF4a2 C106R and HNF4a2 R154X to identify HNF4a regulated genes in kidney cells being several of these genes deregulated in renal cell carcinoma

ABSTRACT: Analysis of gene expression in human embryonic kidney cells HEK293 overexpressing the transcription factors HNF4a2 or mutant forms HNF4a2 C106R and HNF4a2 R154X, which were introduced by FRT/FLP recombination. The analysis was performed 24 hours following 1sg/ml tetracycline treatment to induce the expression of the genes. Results identify HNF4a regulated genes in kidney cells being several of these genes deregulated in renal cell carcinoma.

Note that files GSM52180.txt and GSM52184.txt as downloaded from GEO are identical.

ORGANISM(S): Homo sapiens

SUBMITTER: Ludger Klein-Hitpass

PROVIDER: E-GEOD-2700 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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HNF4alpha reduces proliferation of kidney cells and affects genes deregulated in renal cell carcinoma.

Lucas Belén B Grigo Karen K Erdmann Silke S Lausen Jörn J Klein-Hitpass Ludger L Ryffel Gerhart U GU

Oncogene 20050901 42

Hepatocyte nuclear factor 4alpha (HNF4alpha) is a tissue-specific transcription factor known to regulate a large number of genes in hepatocytes and pancreatic beta cells. Although HNF4alpha is highly expressed in some sections of the kidney, little is known about its role in this organ and about HNF4alpha-regulated genes in the kidney cells. The abundance and activity of HNF4alpha are frequently reduced in renal cell carcinoma (RCC) indicating some tumor suppressing function of HNF4alpha in rena ...[more]

PMID: 16007190

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