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Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls

ABSTRACT: In order to characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we compared gene expression in uncultured amniotic fluid supernatant samples. We identified individually differentially expressed genes via paired t-tests in the matched samples, and a set of differentially expressed genes on chromosome 21 using Gene Set Enrichment Analysis. Functional pathway analysis of the resulting genes highlighted the importance of oxidative stress, ion transport, and G-protein signaling in the DS fetuses. We profiled seven DS expression samples and seven controls matched for gender and approximate gestational age..

ORGANISM(S): Homo sapiens

SUBMITTER: Donna Slonim

PROVIDER: E-GEOD-16176 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses.

Slonim Donna K DK Koide Keiko K Johnson Kirby L KL Tantravahi Umadevi U Cowan Janet M JM Jarrah Zina Z Bianchi Diana W DW

Proceedings of the National Academy of Sciences of the United States of America 20090527 23

To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples. Functional pathway analysis highlighted the importance of oxidative stress, ion transport, and G protein signaling in the DS fetuses. Further evidence supporting these results was derived by correlating the observed gene expression patterns to those of small molecule drugs via the Connectivity ...[more]

PMID: 19474297

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