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The amniotic fluid transcriptome: a source of novel information about human fetal development


ABSTRACT: Amniotic fluid (AF) is a complex biological material that provides a unique window into the developing human. Residual AF supernatant contains cell-free fetal RNA. The objective of this study was to develop an understanding of the AF core transcriptome by identifying the transcripts ubiquitously present in the AF supernatant of euploid midtrimester fetuses. We detected 476 well-annotated genes present in all twelve AF samples. Functional analysis identified 6 physiological systems represented in the AF core transcriptome, including musculoskeletal and nervous system development and function and embryonic/ organismal development. mTOR signaling was identified as a key canonical pathway. Twenty-three highly organ-specific transcripts were identified; six of these are known to be highly expressed by fetal brain. This study demonstrates that AF cell-free fetal RNA can provide biological information about multiple fetal organ systems. This was an in silico investigation of gene expression in 12 euploid midtrimester AF samples acquired primarily for advanced maternal age. We focused on the transcripts called M-bM-^@M-^\presentM-bM-^@M-^] in all of the samples. Functional analyses were performed using Ingenuity software. Fetal-organ specificity was examined for each transcript using the GNF Gene Expression Atlas. For fetal organs not represented in the atlas, manual literature searching and Ingenuity analysis were used.

ORGANISM(S): Homo sapiens

SUBMITTER: Donna Slonim 

PROVIDER: E-GEOD-33168 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The amniotic fluid transcriptome: a source of novel information about human fetal development.

Hui Lisa L   Slonim Donna K DK   Wick Heather C HC   Johnson Kirby L KL   Bianchi Diana W DW  

Obstetrics and gynecology 20120101 1


<h4>Objective</h4>Amniotic fluid is a complex biological material that provides a unique window into the developing human. Residual amniotic fluid supernatant contains cell-free fetal RNA. The objective of this study was to develop an understanding of the amniotic fluid core transcriptome by analyzing the transcripts ubiquitously present in the amniotic fluid supernatant of euploid midtrimester fetuses.<h4>Methods</h4>This was an in silico (computational) investigation using publicly available g  ...[more]

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