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Transcriptional Responses of M. tuberculosis to inhibitors of metabolism

ABSTRACT: Transcriptional profiling of M.tuberculosis was performed using 430 whole-genome microarrays to measure the effects of 75 different drugs, drug combinations or different growth conditions at various times relative to a sample of logarithmically growing MTb. Analysis of this data set revealed 150 underlying clusters of coordinately regulated genes as described in Boshoff et al., 2004. Keywords = Gene clusters Keywords = Mechanism of action Keywords = tuberculosis Keywords = antimycobacterial drugs Keywords = natural product Keywords = respiration

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Timothy Myers

PROVIDER: E-GEOD-1642 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcriptional responses of Mycobacterium tuberculosis to inhibitors of metabolism: novel insights into drug mechanisms of action.

Boshoff Helena I M HI Myers Timothy G TG Copp Brent R BR McNeil Michael R MR Wilson Michael A MA Barry Clifton E CE

The Journal of biological chemistry 20040709 38

The differential transcriptional response of Mycobacterium tuberculosis to drugs and growth-inhibitory conditions was monitored to generate a data set of 430 microarray profiles. Unbiased grouping of these profiles independently clustered agents of known mechanism of action accurately and was successful at predicting the mechanism of action of several unknown agents. These predictions were validated biochemically for two agents of previously uncategorized mechanism, pyridoacridones and phenothia ...[more]

PMID: 15247240

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