Unknown,Transcriptomics,Genomics,Proteomics

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Hybrid wine yeast strains

ABSTRACT: This experiment is the analysis of the transcriptomes of several hybrid yeast strains obtained by crossing natural (from wine) isolates of S. cerevisiae and S. uvarum. All isolations have been done from hybrid strains growing in exponential phase in YPD. Keywords: Strain comparison Transcriptomic analysis of three independent replicates of each yeast strain growing in exponential phase. Each replicate has been hybridized on a different macroarray (F19-F22-F24) as indicated in the sample files. A single DNA (S. cerevisiae 3002 wine strain) genomic hybridization, from the same labeling reaction, was done on the same macroarrays for normalization.

ORGANISM(S): Saccharomyces cerevisiae x Saccharomyces uvarum

SUBMITTER: Lisa Solieri 

PROVIDER: E-GEOD-9888 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mitochondrial inheritance and fermentative : oxidative balance in hybrids between Saccharomyces cerevisiae and Saccharomyces uvarum.

Solieri Lisa L   Antúnez Oreto O   Pérez-Ortín Josè Enrique JE   Barrio Eladio E   Giudici Paolo P  

Yeast (Chichester, England) 20080701 7

Breeding between Saccharomyces species is a useful tool for obtaining improved wine yeast strains, combining fermentative features of parental species. In this work, 25 artificial Saccharomyces cerevisiae x Saccharomyces uvarum hybrids were constructed by spore conjugation. A multi-locus PCR-restriction fragment length polymorphism (PCR-RFLP) analysis, targeting six nuclear gene markers and the ribosomal region including the 5.8S rRNA gene and the two internal transcribed spacers, showed that th  ...[more]

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