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MicroRNA expression data from differentiation of human Cyt49 ESCs into definitive endoderm on MEF feeder layers

ABSTRACT: Pluripotent hESCs can differentiate into the three primary embryonic lineages (endoderm, mesoderm, ectoderm) as well as extraembryonic tissues. Definitive endoderm is the first step into the pathway to endoderm dreived tissues (pancreas, liver, gut, lung) We used microarrays to detail the changes in microRNA expression during the transition from pluripotent hESCs into definitive endoderm hESCs (Cyt49) were differentiated in the presence of Activin A and Wnt3A under low serum conditions to induce DE. formation. Samples were collected at day 0 (hESCs), and day 4 (DE).

ORGANISM(S): Homo sapiens

SUBMITTER: Andrew Hinton

PROVIDER: E-GEOD-16687 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A distinct microRNA signature for definitive endoderm derived from human embryonic stem cells.

Hinton Andrew A Afrikanova Ivka I Wilson Mike M King Charles C CC Maurer Brian B Yeo Gene W GW Hayek Alberto A Pasquinelli Amy E AE

Stem cells and development 20100601 6

Human embryonic stem cells (hESCs) have the potential to differentiate into many adult cell types, and they are being explored as a resource for cell replacement therapies for multiple diseases. In order to optimize in vitro differentiation protocols, it will be necessary to elucidate regulatory mechanisms that contribute to lineage specification. MicroRNAs (miRNAs) are emerging as key regulators of hESC differentiation and embryonic development. In this study, we compare miRNA expression profil ...[more]

PMID: 19807270

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Project description:This SuperSeries is composed of the following subset Series: GSE16678: MicroRNA expression data from differentiation of human Cyt49 ESCs into definitive endoderm in feeder-free conditions GSE16681: mRNA expression data from differentiation of human ESCs into definitive endoderm, Cyt49 on matrigel GSE16687: MicroRNA expression data from differentiation of human Cyt49 ESCs into definitive endoderm on MEF feeder layers GSE16689: MicroRNA expression data from differentiation of human H9 ESCs into definitive endoderm on MEF feeder layers Refer to individual Series

2010-07-30 | E-GEOD-16690 | biostudies-arrayexpress

mRNA expression data from differentiation of human ESCs into definitive endoderm, Cyt49 on matrigel

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