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Microarray Analysis of Gene Expression in Human Normal and Breast Cancer Cells

ABSTRACT: Differential gene expression between normal breast epithelial cells and the malignant counterpart was studied using microarray technique. We utilized GeneAlbum GEM 1-6 from Incyte Pharmaceuticals, Inc., which contains which contains 65,873 cDNA clones (57,172 clones excluding controls), representing 33,515 individual genes. The normal human mammary epithelial cells (HMEC) from 3 donors were used to search different gene expression patterns among individuals, ages and races. In addition, 8 well-established breast cancer cell lines were used as probes against a common normal HMEC from 50 years old donor

ORGANISM(S): Homo sapiens

SUBMITTER: Suxing Liu

PROVIDER: E-GEOD-2155 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Integrative genomics revealed RAI3 is a cell growth-promoting gene and a novel P53 transcriptional target.

Wu Qun Q Ding Wei W Mirza Asra A Van Arsdale Tish T Wei Iris I Bishop W Robert WR Basso Andrea A McClanahan Terri T Luo Lin L Kirschmeier Paul P Gustafson Eric E Hernandez Marco M Liu Suxing S

The Journal of biological chemistry 20050118 13

In this study, differential gene expression between normal human mammary epithelial cells and their malignant counterparts (eight well established breast cancer cell lines) was studied using Incyte GeneAlbum 1-6, which contains 65,873 cDNA clones representing 33,515 individual genes. 3,152 cDNAs showed a > or =3.0-fold expression level change in at least one of the human breast cancer cell lines as compared with normal human mammary epithelial cells. Integration of breast tumor gene expression d ...[more]

PMID: 15659406

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