Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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14028s araC RNA-Seq


ABSTRACT: We used RNA-seq to determine the effects of AraC and arabinose on RNA levels genome-wide in S. enterica. Wild-type or delta araC mutant cells were grown in both the presence and absence of 0.2% L-arabinose. RNA-Seq libraries from two independent biological replicate samples were sequenced for (i) wild-type cells with no treatment, (ii) wild-type cells treated with arabinose, (iii) delta araC cells with no treatment, (iv) delta araC cells treated with arabinose. Comparisons were made between (i) and (ii) [analysis file name: Salmonella RNA-seq processed WT without arabinose vs WT with arabinose.xls], between (i) and (iii) [analysis file name: Salmonella RNA-Seq proessed no arabinose WT vs no arabinose delta araC.xls], and between (ii) and (iv) [analysis file name: Salmonella RNA-Seq processed arabinose WT vs arabinose delta araC.xls]. All analysis files are available from https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-1901/E-MTAB-1901.additional.1.zip

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. 14028s Wu

SUBMITTER: Joseph Wade 

PROVIDER: E-MTAB-1901 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-scale analyses of Escherichia coli and Salmonella enterica AraC reveal noncanonical targets and an expanded core regulon.

Stringer Anne M AM   Currenti Salvatore S   Bonocora Richard P RP   Baranowski Catherine C   Petrone Brianna L BL   Palumbo Michael J MJ   Reilly Andrew A AA   Zhang Zhen Z   Erill Ivan I   Wade Joseph T JT  

Journal of bacteriology 20131122 3


Escherichia coli AraC is a well-described transcription activator of genes involved in arabinose metabolism. Using complementary genomic approaches, chromatin immunoprecipitation (ChIP)-chip, and transcription profiling, we identify direct regulatory targets of AraC, including five novel target genes: ytfQ, ydeN, ydeM, ygeA, and polB. Strikingly, only ytfQ has an established connection to arabinose metabolism, suggesting that AraC has a broader function than previously described. We demonstrate  ...[more]

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