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NCI-H1299 cell line transfected with human DENND2D and vector

ABSTRACT: Gene expression for NCI-H1299 cell line transfected with human DENND2D and vector (pcDNA3.1/V5-His TOPO TA vector) respectively. The microarray experiment was designed to perform four replicates for each H1299-DENND2D and H1299-vector sample. cRNA used in replication 1 and 2 was from the same label reaction to perform the hybridization replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Hang Liu

PROVIDER: E-GEOD-16890 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Power of deep sequencing and agilent microarray for gene expression profiling study.

Feng Lin L Liu Hang H Liu Yu Y Lu Zhike Z Guo Guangwu G Guo Suping S Zheng Hongwei H Gao Yanning Y Cheng Shujun S Wang Jian J Zhang Kaitai K Zhang Yong Y

Molecular biotechnology 20100601 2

Next-generation sequencing-based Digital Gene Expression tag profiling (DGE) has been used to study the changes in gene expression profiling. To compare the quality of the data generated by microarray and DGE, we examined the gene expression profiles of an in vitro cell model with these platforms. In this study, 17,362 and 15,938 genes were detected by microarray and DGE, respectively, with 13,221 overlapping genes. The correlation coefficients between the technical replicates were >0.99 and the ...[more]

PMID: 20432071

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