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Transcription profiling of Drosophila pooled Malpighian tubules vs. whole fly

ABSTRACT: This is an affymetrix array experiment comparing the transcriptome of the Malpighian tubule (or renal tubule) of 7-day adult Oregon R strain Drosophila melanogaster with matched whole fly samples. It is described in:,Wang, J., Kean, L., Yang, J., Allan, A. K., Davies, S. A., Herzyk, P. and Dow, J. A. T. (2004). Function-informed transcriptome analysis of Drosophila renal tubule. Genome Biol. In press.,There are five tubule samples (each derived from approx 1000 tubules (!)), and 5 matched whole-fly samples. i.e. tubule 2 is dissected from the same vial as WholeFly2.,As the tubule is probably the premier tissue for true physiology in Drosophila, the dataset can usefully be interrogated in conjunction with the detailed physiological understanding of the tissue: see,http://fly.to/tubules

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Julian Dow

PROVIDER: E-GEOD-1690 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Function-informed transcriptome analysis of Drosophila renal tubule.

Wang Jing J Kean Laura L Yang Jingli J Allan Adrian K AK Davies Shireen A SA Herzyk Pawel P Dow Julian A T JA

Genome biology 20040826 9

<h4>Background</h4>Comprehensive, tissue-specific, microarray analysis is a potent tool for the identification of tightly defined expression patterns that might be missed in whole-organism scans. We applied such an analysis to Drosophila melanogaster Malpighian (renal) tubule, a defined differentiated tissue.<h4>Results</h4>The transcriptome of the D. melanogaster Malpighian tubule is highly reproducible and significantly different from that obtained from whole-organism arrays. More than 200 gen ...[more]

PMID: 15345053

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