Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of Drosophila melanogaster Malpighian tubule v whole fly

ABSTRACT: This is an affymetrix array experiment comparing the transcriptome of the Malpighian tubule (or renal tubule) of 7-day adult Oregon R strain Drosophila melanogaster with matched whole fly samples. There are five tubule samples (each derived from approx 1000 tubules (!)), and 5 matched whole-fly samples. i.e. tubule 2 is dissected from the same vial as WholeFly2. As the tubule is probably the premier tissue for true physiology in Drosophila, the dataset can usefully be interrogated in conjunction with the detailed physiological understanding of the tissue: see http://fly.to/tubules

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE1690 | GEO | 2004/08/24

SECONDARY ACCESSION(S): PRJNA90651

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of Drosophila pooled Malpighian tubules vs. whole fly

Project description:This is an affymetrix array experiment comparing the transcriptome of the Malpighian tubule (or renal tubule) of 7-day adult Oregon R strain Drosophila melanogaster with matched whole fly samples. It is described in:,Wang, J., Kean, L., Yang, J., Allan, A. K., Davies, S. A., Herzyk, P. and Dow, J. A. T. (2004). Function-informed transcriptome analysis of Drosophila renal tubule. Genome Biol. In press.,There are five tubule samples (each derived from approx 1000 tubules (!)), and 5 matched whole-fly samples. i.e. tubule 2 is dissected from the same vial as WholeFly2.,As the tubule is probably the premier tissue for true physiology in Drosophila, the dataset can usefully be interrogated in conjunction with the detailed physiological understanding of the tissue: see,http://fly.to/tubules

2007-07-06 | E-GEOD-1690 | biostudies-arrayexpress

Malpighian tubules (MT) gene expression between control and yki3SA gut-tumor-bearing flies.

Project description:To investigate the function of Malpighian tubules on host wasting, we established a fly model of tumor-induced wasting by activation of an oncogene Yki in adult intestinal stem cells (ISCs) for 8 days to cause gut-tumor formation and systemic organ wasting and metabolic abnormalities. We then performed RNA-seq and gene expression analysis in Malpighian tubules from control and yki3SA tumor-bearing flies

2022-05-29 | GSE204866 | GEO

Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation

Project description:Canine heartworm is a widespread and potentially fatal mosquito-borne disease caused by infections with the parasitic nematode, Dirofilaria immitis. We have previously shown that systemic activation of the Toll immune pathway via silencing of the negative regulator Cactus in Aedes aegypti blocks parasite development in the Malpighian tubules, the mosquito renal organ. However, it was not established whether the Malpighian tubules were directly responding to Toll activation or were alternatively responding to upregulated proteins or other changes to the hemolymph driven by other tissues. Distinguishing these possibilities is crucial for developing more precise strategies to block D. immitis while potentially avoiding the fitness cost to the mosquito associated with Cactus silencing. This study defines the transcriptional response of Ae. aegypti Malpighian tubules after systemic Toll activation via intra-thoracic injection of dsCactus and found, like the response of whole mosquitoes, a significant increase in expression of Toll pathway target genes. Additionally, we identified a significant overlap between the transcriptional response of the Malpighian tubules and proteins upregulated in the hemolymph. Our data show that Malpighian tubules are capable of RNAi-mediated gene silencing and directly respond to dsCactus treatment by upregulating canonical Toll pathway targets. Though not definitive, the strong correspondence between the Malpighian tubule transcriptional and the hemolymph proteomic responses provides evidence that the tubules may contribute to mosquito humoral immunity.

2024-08-27 | PXD036225 | Pride

Difference of gene expression in Malpighian tubules (MTs) betweeen control, "yki3SA" tumor, and "yki3SA+ITP-i" tumor-bearing flies

Project description:To investigate the function of malpighian tubule in tumor-induced host wasting, we established a wasting model in fly by activation of Yki in adult midgut ISCs, in which the host flies present the sysytmeic organ wasting and metabolic abnormalities.

2023-08-11 | GSE240166 | GEO

Transcription profiling of adult drosophila renal tubule vs whole fly

Project description:The experiment was designed to identify genes abundant or enriched in adult Drosophila melanogaster Malpighian (renal) tubule as compared to the whole fly.

2004-06-23 | E-MEXP-127 | biostudies-arrayexpress

Natural variation affecting allele-specific expression in the Malpighian tubules of Drosophila melanogaster

Project description:We used RNA-seq to investigate natural variation in gene expression in the Malpighian tubules of three inbred Drosophila melanogaster strains and their F1 hybrids. One of the strains was from a population in the species’ ancestral range (Zambia), while the other two were from a more recently derived population (Sweden).

2018-08-29 | GSE103645 | GEO

Transcription profing of Drosophila tissues in adult and larva

Project description:A new online resource (flyatlas.org) provides the most comprehensive view yet of expression in multiple tissues of Drosophila. Meta-analysis of the data reveals that a significant fraction ofthe genome is expressed with great tissue specificity in the adult, demonstrating the need for the functional genomic community to embrace a wider range of functional phenotypes. Well-known developmental genes are often re-used in surprising tissues in the adult, suggesting new functions. The homologues of many human genetic disease loci show selective expression in Drosophila tissues with function analogous to the affected tissues in the cognate human disease, providing a useful filter for potential candidate genes. Experiment Overall Design: FlyAtlas is composed of data covering 8 distinct adult tissues (brain, head, midgut, Malpighian tubules,hindgut, testis, ovary, male accessory glands) so far, together with two larval tissues (tubule, fat body), each run with 4 replicates on Affymetrix Dros Genome 2 chips (with probesets for 18770 transcripts), and compared with a matched whole-fly sample.

2008-03-13 | E-GEOD-7763 | biostudies-arrayexpress

Transcriptional and functional motifs defining renal function revealed by single nuclear sequencing

Project description:Like humans, insects rely on precise regulation of their internal environments to survive. The insect renal system consists of Malpighian tubules and nephrocytes that share similarities to the mammalian kidney. Studies of the adult Drosophila Malpighian tubules and nephrocytes have led to many insights in our understanding of stem cell regeneration, protein reabsorption, excretion of waste products, and human kidney diseases. Here, we analyzed single-nucleus RNA sequencing (snRNA-seq) data sets to characterize the cell types of the adult male and female fly kidney.

2022-05-11 | GSE202575 | GEO

Population- and sex-biased gene expression in the excretion organs of Drosophila melanogaster

Project description:We used RNA-seq to investigate gene expression variation in Malpighian tubules, which have a function analogous to that of human kidneys. In order to characterize population differentiation, we sequenced the Malpighian tubule transcriptomes of flies derived from two populations, one from sub-Saharan Africa (Zimbabwe) and one from Europe (the Netherlands). Males and females were examined separately. Overall, we found a high amount of differential expression between sexes (2,308 genes) and populations (2,474 genes). Although most of the differentially expressed genes were consistent between sexes and populations, there were 615 genes showed sex-biased expression in only one population and 557 genes showed population-biased expression in only one sex. mRNA expression profiles of Drosophila melanogaster Malpighian tubules from adult males and females from a European and an African population (2 biological replicates per sex and population)

2014-09-25 | E-GEOD-58578 | biostudies-arrayexpress