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Proteomic analysis of microdissected renal tubules

ABSTRACT: Kidney is a vital organ which help maintain body homeostasis. Three related processes including glomerular filtration, tubular reabsorption and secretion are precisely regulated in the kidney in response to changes in the amount of both water and various solutes. To understand how the kidney can achieve this challenging goal, it is important to know the protein composition in the kidney. However, unlike other solid organs, kidney has a very intricate architecture, including at least 14 distinct tubule segments. This reason limits the interpretibility of experiments perfomred using whole kidney homegenate samples. To mitigate this problem, we combined kidney tubule microdissection technique with protein mass spectrometry to study the proteome of 14 individual rat kidney tubule segment.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Kidney

DISEASE(S): Disease Free

SUBMITTER: Kavee Limbutara

LAB HEAD: Mark A. Knepper

PROVIDER: PXD016958 | Pride | 2020-04-20

REPOSITORIES: pride

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			Action	DRS
	CCD0808_F1.raw	Raw
	CCD0808_F2.raw	Raw
	CCD0808_F3.raw	Raw
	CCD0808_F4.raw	Raw
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Publications

Quantitative Proteomics of All 14 Renal Tubule Segments in Rat.

Limbutara Kavee K Chou Chung-Lin CL Knepper Mark A MA

Journal of the American Society of Nephrology : JASN 20200501 6

<h4>Background</h4>Previous research has used RNA sequencing in microdissected kidney tubules or single cells isolated from the kidney to profile gene expression in each type of kidney tubule epithelial cell. However, because proteins, not mRNA molecules, mediate most cellular functions, it is desirable to know the identity and amounts of each protein species to understand function. Recent improvements in the sensitivity of mass spectrometers offered us the ability to quantify the proteins expre ...[more]

PMID: 32358040

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