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Transcriptional analysis of Leishmania major methotrexate resistant strains using full-genome DNA microarrays

ABSTRACT: Transcriptional analysis of the drug resistant strain L. major MTX60.4 compared to the wild-type strain LV39 The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.major LV39 Keywords: drug resistance Two-condition experiment, resistant strain MTX60.4 vs wild-type LV39. Four biological replicates for each strain, independently grown and harvested. One replicate per array

ORGANISM(S): Leishmania major

SUBMITTER: J Ubeda

PROVIDER: E-GEOD-9948 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy.

Ubeda Jean-Michel JM Légaré Danielle D Raymond Frédéric F Ouameur Amin Ahmed AA Boisvert Sébastien S Rigault Philippe P Corbeil Jacques J Tremblay Michel J MJ Olivier Martin M Papadopoulou Barbara B Ouellette Marc M

Genome biology 20080718 7

<h4>Background</h4>Drug resistance can be complex, and several mutations responsible for it can co-exist in a resistant cell. Transcriptional profiling is ideally suited for studying complex resistance genotypes and has the potential to lead to novel discoveries. We generated full genome 70-mer oligonucleotide microarrays for all protein coding genes of the human protozoan parasites Leishmania major and Leishmania infantum. These arrays were used to monitor gene expression in methotrexate resist ...[more]

PMID: 18638379

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