Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human multiple myeloma cells ( MM1.S) cells with control or beta-catenin knockdown.

ABSTRACT: MM1.S cells stably transduced with control or beta-catenin shRNA were established. Total RNA was isolated from 5x 10^6 cells of each in triplicate. Experiment Overall Design: Comparative gene expression profiling in multiple myeloma.

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel Ruben Carrasco

PROVIDER: E-GEOD-17385 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression.

Dutta-Simmons Jui J Zhang Yunyu Y Gorgun Gullu G Gatt Moshe M Mani Mala M Hideshima Teru T Takada Kohichi K Carlson Nicole E NE Carrasco Daniel E DE Tai Yu-Tzu YT Raje Noopur N Letai Anthony G AG Anderson Kenneth C KC Carrasco Daniel R DR

Blood 20090803 13

Multiple myeloma (MM) is a cancer of plasma cells with complex molecular characteristics that evolves from monoclonal gammopathy of undetermined significance, a highly prevalent premalignant condition. MM is the second most frequent hematologic cancer in the United States, and it remains incurable, thereby highlighting the need for new therapeutic approaches, particularly those targeting common molecular pathways involved in disease progression and maintenance, shared across different MM subtype ...[more]

PMID: 19652203

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Project description:The microbial loop is the conventional model by which nutrients and minerals are recycled in aquatic eco-systems. Biochemical pathways in different organisms become metabolically inter-connected such that nutrients are utilized, processed, released and re-utilized by others. The result is that unrelated individuals end up impacting each others’ fitness directly through their metabolic activities. This study focused on the impact of programmed cell death (PCD) on a population’s growth as well as its role in the exchange of carbon between two naturally co-occurring halophilic organisms. Flow cytometric, biochemical, 14C radioisotope tracing assays, and global transcriptomic analyses show that organic algal photosynthate released by Dunalliela salina cells undergoing PCD complements the nutritional needs of other non-PCD D. salina cells. This occurs in vitro in a carbon limited environment and enhances the growth of the population. In addition, a co-occurring heterotroph Halobacterium salinarum re-mineralizes the carbon providing elemental nutrients for the mixoheterotrophic chlorophyte. The significance of this is uncertain and the archaeon can also subsist entirely on the lysate of apoptotic algae. PCD is now well established in unicellular organisms; however its ecological relevance has been difficult to decipher. In this study we found that PCD in D. salina causes the release of organic nutrients such as glycerol, which can be used by others in the population as well as a co-occurring halophilic archaeon. H. salinarum also re-mineralizes the dissolved material promoting algal growth. PCD in D. salina was the mechanism for the flow of dissolved photosynthate between unrelated organisms. Ironically, programmed death plays a central role in an organism’s own population growth and in the exchange of nutrients in the microbial loop. Halobacterium NRC-1 was grown to mid-logarithmic phase (OD600 ~0.4 – 0.8) in MM2. Cells were harvested and washed in MM1 and incubated in MM1 (control) or in Dunaliella harvested supernatant. Cells were harvested at time zero, 5, 10, 20, 40, 80, 160 minutes. RNA from two biological replicate time courses were prepared, averages of these replicates are reported in the published study. The zero time point was harvested immediately after incubation Halobacteria-NRC1 in MM1 or in Dunaliella salina's supernatant.

Dataset Information

Transcription profiling of human multiple myeloma cells ( MM1.S) cells with control or beta-catenin knockdown.

Publications

Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression.

Similar Datasets

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