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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Identification of beta-catenin-associated RNAs in myeloid leukaemia cells through RIP-seq

ABSTRACT: Myeloid cell lines (K562 and HEL) were treated overnight with CHIR99021 or a vehicle control (DMSO). CHIR99021 treatment inhibits GSK3B within the destruction complex functioning in Wnt/beta-catenin signalling pathway, thereby preventing beta-catenin degradation and promoting its stabilization. Following the overnight incubation, beta-catenin RIP (RNA immunoprecipitation) was performed in both K562 and HEL cells. RNA samples obtained from beta-catenin RIP in these cells were then sequenced to identify beta-catenin-associated RNAs under CHIR99021 treatment compared to basal conditions (DMSO control).

INSTRUMENT(S): NextSeq 500, 75 bp single end lane, NextSeq 500

ORGANISM(S): Homo sapiens

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PROVIDER: E-MTAB-14675 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP166929

REPOSITORIES: biostudies-arrayexpress

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Characterisation of the Beta-Catenin cytoplasmic/nuclear interactome in leukaemia cells

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