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Minimal metabolic pathway structure is consistent with associated macromolecular interactions.

ABSTRACT: We sequenced mRNA of three dual perturbation experiments of E. coli K12 MG1655 changing both genetic and environmental conditions. Each dual perturbation experiment consists of four RNA-seq samples (WT, WT+nutrient supplementation, transcription factor KO, transcription factor KO+nutrient supplementation). The three conditions are: 1) adenine supplementation/nac KO, 2) L-tryptophan supplementation/cra KO, 3) anaerobic/mntR KO. Determination of whether a transcription factor plays a regulatory role in a particular environmental shift by examining differential expression of mRNA levels.

ORGANISM(S): Escherichia coli K-12

SUBMITTER: Bernhard Palsson

PROVIDER: E-GEOD-48324 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Minimal metabolic pathway structure is consistent with associated macromolecular interactions.

Project description:We sequenced mRNA of three dual perturbation experiments of E. coli K12 MG1655 changing both genetic and environmental conditions. Each dual perturbation experiment consists of four RNA-seq samples (WT, WT+nutrient supplementation, transcription factor KO, transcription factor KO+nutrient supplementation). The three conditions are: 1) adenine supplementation/nac KO, 2) L-tryptophan supplementation/cra KO, 3) anaerobic/mntR KO.

2014-06-30 | GSE48324 | GEO

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