Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression data of BBB and BCB two-cell embryos


ABSTRACT: We constructed one-cell stage embryos by maternal pronuclear (mPN) transfer having B6 ooplasm, B6 paternal PN (pPN), and either B6 or C3H mPN (BBB and BCB, respectively). We collected embryos of each type that were either treated (BBB+a, BCB+a) or untreated with α-amanitin (BBB, BCB) at the two-cell stage for microarray analysis. Comparison of the transcriptomes of these different kinds of embryos revealed genes for which expression differs according to maternal PN strain of origin, and the α-amanitin data revealed which of these differences is due to gene transcription, as opposed to any transcription-independent differences attributable to ooplasm-derived maternal mRNA pools. There are 4 replicates for each kind/treatment two-cell embryos (BBB, BCB, BBB+a, BCB+a).

ORGANISM(S): Mus musculus

SUBMITTER: Rita Vassena 

PROVIDER: E-GEOD-17886 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Early transcription from the maternal genome controlling blastomere integrity in mouse two-cell-stage embryos.

Han Zhiming Z   Mtango Namdori R NR   Zhong Zhisheng Z   Vassena Rita R   Latham Keith E KE  

American journal of physiology. Cell physiology 20100127 5


Blastomere cytofragmentation in mammalian embryos poses a significant problem in applied and clinical embryology. Mouse two-cell-stage embryos display strain-dependent differences in the rate of cytofragmentation, with a high rate observed in C3H/HeJ embryos and a lower rate observed in C57BL/6 embryos. The maternally inherited genome exerts the strongest effect on the process, with lesser effects mediated by the paternally inherited genome and the ooplasm. The effect of the maternal genome is t  ...[more]

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