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Transcription profiling of Arabidopsis early anther

ABSTRACT: We performed microarray experiments with RNAs from dissected immature anthers (stages 4-7) from wild-type, dyt1 and ams mutant plants, using the Affymetrix ATH1 chip. Experiment Overall Design: Anthers from Arabidopsis thaliana were selected at 4-7 stages of early development for RNA extraction and hybridization on Affymetrix microarrays. Samples from two mutants and wild-type anther.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Xuan Ma

PROVIDER: E-GEOD-18225 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of the Arabidopsis anther transcriptome by DYT1 for pollen development.

Feng Baomin B Lu Dihong D Ma Xuan X Peng Yiben Y Sun Yujin Y Ning Gang G Ma Hong H

The Plant journal : for cell and molecular biology 20120920 4

Several genes encoding transcription factors have been shown to be essential for male fertility in plants, suggesting that transcriptional regulation is a major mechanism controlling anther development in Arabidopsis. DYSFUNCTIONAL TAPETUM 1 (DYT1), a putative bHLH transcription factor, plays a critical role in regulating tapetum function and pollen development. Here, we compare the transcriptomes of young anthers of wild-type and the dyt1 mutant, demonstrating that DYT1 is upstream of at least ...[more]

PMID: 22775442

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Project description:This study was initiated with the objective of identifying the anther/tapetum specific promoters from cotton floral buds. Cotton is an important commercial crop. Hybrid cotton varieties are developed to obtain improved yield and fiber quality. Most of the hybrid seed production in cotton is carried out by hand emasculation, which requires large amount of manpower, resulting in high cost of hybrid seed. We are developing barnase-barstar based male sterility system, which would be a better alternative for hybrid development. The tapetum specific promoters are main requirement for such a system. The study was thus carried out to identify genes expressed in the anthers. Cotton bud sizes were correlated with tapetum development. RNA was isolated from following tissues: • Anther tissues from buds at pre-meiotic stage of development (Tapetum absent) • Buds without anther tissues at pre-meiotic stage of development • Anther tissues from buds during meiosis (Tapetum present) • Buds without anther tissues during meiosis • Anther tissues from buds at post-meiotic stage of development (Tapetum degenerated) • Buds without anther tissues at post-meiotic stage of development • Leaf tissues • Seedling 5 days after germination Biotin labeled cRNA was hybridized on Affymertix cotton Genechip Genome array following Affymetrix protocols. Three biological replicates were maintained.

2013-10-31 | E-GEOD-51779 | biostudies-arrayexpress

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2013-05-01 | E-GEOD-43982 | biostudies-arrayexpress

micro-proteomics experiment for single meiotic-phase barley anthers

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2013-07-28 | E-GEOD-43531 | biostudies-arrayexpress

The proteome of developing barley anthers during meiotic prophase I

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Expression data from early anther

Project description:We performed microarray experiments with RNAs from dissected immature anthers (stages 4-7) from wild-type, dyt1 and ams mutant plants, using the Affymetrix ATH1 chip.

2009-09-23 | GSE18225 | GEO

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Transcriptome analysis of multiple pre-meiotic anther stages and laser microdissected cell types in maize

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2005-10-01 | GSE2619 | GEO

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