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Unknown,Transcriptomics,Genomics,Proteomics

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HepaRG cells as a model of the primary human hepatocyte transcriptome

ABSTRACT: The aim of this experiment is to determine the similarities and differences between gene expression profiles in HepaRG cells versus primary human hepatocytes, human liver, and the commonly used HepG2 cell. We compared the gene expression profiles from replicate triplicate biological samples of human liver, primary human hepatocytes, HepG2 cells, and HepaRG at differing levels of maturity (differentiated and undifferentiated).

ORGANISM(S): Homo sapiens

SUBMITTER: Steven Hart

PROVIDER: E-GEOD-18269 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A comparison of whole genome gene expression profiles of HepaRG cells and HepG2 cells to primary human hepatocytes and human liver tissues.

Hart Steven N SN Li Ye Y Nakamoto Kaori K Subileau Eva-anne EA Steen David D Zhong Xiao-bo XB

Drug metabolism and disposition: the biological fate of chemicals 20100312 6

HepaRG cells, derived from a female hepatocarcinoma patient, are capable of differentiating into biliary epithelial cells and hepatocytes. More importantly, differentiated HepaRG cells are able to maintain activities of many xenobiotic-metabolizing enzymes, and expression of the metabolizing enzyme genes can be induced by xenobiotics. The ability of these cells to express and induce xenobiotic-metabolizing enzymes is in stark contrast to the frequently used HepG2 cells. The previous studies have ...[more]

PMID: 20228232

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HepaRG cells as a model of the primary human hepatocyte transcriptome

Project description:The aim of this experiment is to determine the similarities and differences between gene expression profiles in HepaRG cells versus primary human hepatocytes, human liver, and the commonly used HepG2 cell.

2009-12-29 | GSE18269 | GEO

Proteome and secretome of HepaRG cells

Project description:Primary human hepatocytes, the gold standard for in vitro studies of liver-related functions, suffer from uncertain availability and high variability in cell activity. Over years, a number of alternative hepatic cell lines have lost major liver-like functions, but not HepaRG cells. Therefore, their increasing use worldwide today arouses the need for establishing a reference functional status of differentiated HepaRG cells known as HPR116, which originate from the initial cell bank. Deep proteome and secretome analyses enabled us to show that they nicely express, at levels generally close to those in primary hepatocytes, master regulators of the hepatic phenotype, structural elements that ensure liver-like polarisation and factors supporting their transdifferentiation properties. Their highly differentiated status, mitochondrial functionality, hepatokine secretion ability and response to insulin was verified. Overall, the HepaRG cell system appears as robust surrogate cell system to primary hepatocytes, versatile enough to study not only xenobiotic detoxification but also the control of hepatic energy metabolism, secretory function and disease-related signalling pathways.

2019-02-27 | PXD010304 | Pride

Characterization of gene expression patterns among PXB-cells, cryopreserved primary human hepatocytes, HepaRG cells, HepG2 cells, and total human liver tissue

Project description:Here we provide the gene expression patterns of PXB-cells , primary human hepatocytes (PHH), and HepaRG cells in Matrigel sandwich culture, HepG2 cells in 2D culture, and total human liver tissues. These data will provide insight into the appropriate selection of the cells for in vitro cellular models.

2020-02-28 | GSE146023 | GEO

Inter-individual variability in gene expression profiles in human hepatocytes and comparison with HepaRG cells

Project description:Basal transcriptomic profiling of primary human hepatocytes and HepaRG cells We have analysed basal gene expression profiles in 6 primary human hepatocytes (2 technical replicates) and in 2 HepaRG cell passages (3 batches, 4 technical replicates)

2015-10-01 | GSE41289 | GEO

Effect of Microcystin-LR and cylindrospermopsin (CYN) on gene expression in human hepatocytes, HepaRG cells and HCC HepG2 cells.

Project description:Human hepatocytes, HepaRG cells and HCC HepG2 cells were treated with 50nM of Microcystin-LR and cylindrospermopsin (CYN) to observe changes in gene expression.

2023-05-12 | E-MTAB-12881 | biostudies-arrayexpress

System Analysis of The Functional Cross-Talk Between PPARα, LXR and FXR in HepaRG Liver Cells

Project description:The purpose of the present study was to explore in liver cells the connectivity that operates between three nuclear receptors in the liver, LXR, FXR, and PPARa, all three known to act on lipid and glucose metabolism, and also on inflammation. The human cell line HepaRG was selected for its proximity to human primary hepatocytes. Global gene expression of differentiated HepaRG cells was assessed after 4 hours and 24 hours of exposure to GW3965 (LXR agonist), GW7647 (PPARα agonist), and GW4064 and CDCA (FXR synthetic and natural agonist, respectively).

2019-06-27 | GSE124053 | GEO

Hepatocytes-HepaRG reprogramming.

Project description:To identify the molecular mechanisms and environmental inducers contributing to reprogramming of hepatocytes into progenitors in HCC context, we used the HepaRG cell line as model. We performed transcriptomic analysis at various time-points during this process; 0h, 1h, 4h, 8h, 12h, 16h, 24h and 48h.

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Human Hepatocyte Proteome

Project description:Here we report a comprehensive proteomic analysis of purified human hepatocytes and a human hepatoma cell line HepG2. The complete dataset comprises 9,400 proteins and provides quantitative depiction of the proteomes of hepatocytes and HepG2 cells at the protein titer and copy number dimensions.

2016-03-07 | MSV000079562 | MassIVE

Hepatocytes-HepaRG reprogramming.

2013-12-05 | GSE52989 | GEO

Gene expression data from acetaminophen-induced toxicity in human hepatic in vitro systems and clinical liver samples

Project description:In this study we conducted transcriptomics analyses of: (i) liver samples from patients suffering from acetaminophen-induced acute liver failure (n=3) and from healthy livers (n=2) and (ii) hepatic cell systems exposed to acetaminophen, including their respective vehicle controls. The investigated in vitro systems are: HepaRG cells, HepG2 cells and a novel human skinpostnatal stem cell-derived model i.e. human skin-precursors-derived hepatocyte-like cells (hSKP-HPC). Clinical samples were obtained after surgical removal of the explant liver of patients diagnosed with ALF due to acetaminophen intoxication and treated by orthotopic liver transplantation (n=3). Samples from healthy livers were obtained from individuals deceased from brain damage (n=2). Different samples of hSKP, HepG2 and HepaRG were obtained from the same cell batch of each cell system. All cells were exposed for 24 hours to their corresponding sub cytotoxic concentrations (IC10): IC10(hSKP-HPC)=18mM; IC10(HepaRG)=13mM; IC10(HepG2)=2mM. Experiments were conducted in triplicate. This dataset is part of the TransQST collection.

2015-11-04 | E-GEOD-74000 | biostudies-arrayexpress

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