Proteomics

Dataset Information

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Proteome and secretome of HepaRG cells


ABSTRACT: Primary human hepatocytes, the gold standard for in vitro studies of liver-related functions, suffer from uncertain availability and high variability in cell activity. Over years, a number of alternative hepatic cell lines have lost major liver-like functions, but not HepaRG cells. Therefore, their increasing use worldwide today arouses the need for establishing a reference functional status of differentiated HepaRG cells known as HPR116, which originate from the initial cell bank. Deep proteome and secretome analyses enabled us to show that they nicely express, at levels generally close to those in primary hepatocytes, master regulators of the hepatic phenotype, structural elements that ensure liver-like polarisation and factors supporting their transdifferentiation properties. Their highly differentiated status, mitochondrial functionality, hepatokine secretion ability and response to insulin was verified. Overall, the HepaRG cell system appears as robust surrogate cell system to primary hepatocytes, versatile enough to study not only xenobiotic detoxification but also the control of hepatic energy metabolism, secretory function and disease-related signalling pathways.

INSTRUMENT(S): amaZon ETD, impact HD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Fabrice BERTILE  

LAB HEAD: Sarah Cianférani

PROVIDER: PXD010304 | Pride | 2019-02-27

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
00046TG_1-d2_01_218.d.zip Other
00048TG_1-d3_01_220.d.zip Other
00050TG_1-e2_01_222.d.zip Other
00052TG_1-c2_01_224.d.zip Other
00054TG_1-d4_01_226.d.zip Other
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Publications

In-Depth Proteome Analysis Highlights HepaRG Cells as a Versatile Cell System Surrogate for Primary Human Hepatocytes.

Tascher Georg G   Burban Audrey A   Camus Sandrine S   Plumel Marine M   Chanon Stéphanie S   Le Guevel Remy R   Shevchenko Valery V   Van Dorsselaer Alain A   Lefai Etienne E   Guguen-Guillouzo Christiane C   Bertile Fabrice F  

Cells 20190221 2


Of the hepatic cell lines developed for in vitro studies of hepatic functions as alternatives to primary human hepatocytes, many have lost major liver-like functions, but not HepaRG cells. The increasing use of the latter worldwide raises the need for establishing the reference functional status of early biobanked HepaRG cells. Using deep proteome and secretome analyses, the levels of master regulators of the hepatic phenotype and of the structural elements ensuring biliary polarity were found t  ...[more]

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