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Role of Cryptococcus neoformans Sre1-pathway in low oxygen gene expression

ABSTRACT: Comparison of wild-type serotype A C. neoformans cells grown at 21% and 3% oxygen for 2 hours in rich medium. Also comparison of wild-type and Sre1-pathway mutant strains grown at 3 % oxygen for 2 hours in rich medium. Two-condition experiment. Each comparison was performed in two biological replicates, each containing duplicate probes/array (=4 data points total per comparison).

ORGANISM(S): Filobasidiella neoformans

SUBMITTER: June Kwon-chung

PROVIDER: E-GEOD-18282 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cryptococcus neoformans Site-2 protease is required for virulence and survival in the presence of azole drugs.

Bien Clara M CM Chang Yun C YC Nes W David WD Kwon-Chung Kyung J KJ Espenshade Peter J PJ

Molecular microbiology 20091008 3

In the human fungal pathogen Cryptococcus neoformans, the SREBP orthologue Sre1 is important for adaptation and growth in nutrient-limiting host tissues. In this study, we characterize the C. neoformans serotype A Sre1 and its activating protease, Stp1. We demonstrate that Stp1 is a functionally conserved orthologue of the mammalian Site-2 protease and that Stp1 cleaves Sre1 within its predicted first transmembrane segment. Gene expression analysis revealed that Stp1 is required for both Sre1-de ...[more]

PMID: 19818023

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