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Gene expression signatures for mESC treated by RNAi strategy on down regulation of gene Mtf2

ABSTRACT: To further explore the function of Mtf2, the homolog of Drosophila Polycomb group gene PCL, we have employed the mouse whole genome microarray expression profiling as a discovery platform to identify genes mediated by Mtf2. Oligos of 2 different RNAi target sites on gene Mtf2 were cloned into RNAi Vector pSuper and transfected individually into Mouse Embryonic Stem Cell line E14. Empty Vector pSuper and plasmid containing RNAi target against gene Luciferase (Firefly) were performed in the same way as negative controls. The down regulation effect of gene Mtf2 was quantified by real-time PCR in the same RNA samples subjected to microarray analysis. mESC was transfected by RNAi plasmids with resistence of puromycin and selected by Puromycin(1mg/ml) for 2 days. 4 independent experiments were performed for vector pSuper, plasimd pSuper-Luci-RNAi, pSuper-Mtf2-RNAi-1, pSuper-Mtf2-RNAi-3.

ORGANISM(S): Mus musculus

SUBMITTER: Dan Xie 

PROVIDER: E-GEOD-18319 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rewirable gene regulatory networks in the preimplantation embryonic development of three mammalian species.

Xie Dan D   Chen Chieh-Chun CC   Ptaszek Leon M LM   Xiao Shu S   Cao Xiaoyi X   Fang Fang F   Ng Huck H HH   Lewin Harris A HA   Cowan Chad C   Zhong Sheng S  

Genome research 20100310 6

Mammalian preimplantation embryonic development (PED) is thought to be governed by highly conserved processes. While it had been suggested that some plasticity of conserved signaling networks exists among different mammalian species, it was not known to what extent modulation of the genomes and the regulatory proteins could "rewire" the gene regulatory networks (GRN) that control PED. We therefore generated global transcriptional profiles from three mammalian species (human, mouse, and bovine) a  ...[more]

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