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Expression profiling of hypoxic HepG2 hepatoma, U87 glioma, and MDA-MB231 breast cancer cells: time course

ABSTRACT: Analysis of expression changes of cultured HepG2 hepatoma, U87 glioma, and MDA-MB231 breast cancer cells subjected to hypoxia (0.5% O2) for 0, 4, 8, 12 hours . Results provide insight to cell type-specific response to hypoxia. HepG2 hepatoma, U87 glioma, and MDA-MB231 breast cancer cells were collected under normoxic conditions (~19% O2, 0 hours) and after 4, 8 and 12 hours of hypoxia treatment (0.5% O2). For each cell line, three replicates of total RNA at each time point were prepared using Trizol and submitted to the DFCI Microarray Core for labeling, hybridization to Affymetrix HG-U133Plus2 oligonucleotide arrays and image scanning.

ORGANISM(S): Homo sapiens

SUBMITTER: Xiaobo Xia 

PROVIDER: E-GEOD-18494 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia.

Xia Xiaobo X   Kung Andrew L AL  

Genome biology 20091014 10

<h4>Background</h4>Hypoxia-inducible factor 1 (HIF-1) plays a key role in cellular adaptation to hypoxia. To better understand the determinants of HIF-1 binding and transactivation, we used ChIP-chip and gene expression profiling to define the relationship between the epigenetic landscape, sites of HIF-1 binding, and genes transactivated by hypoxia in two cell lines.<h4>Results</h4>We found that when cells were acutely subjected to hypoxia, HIF-1 preferentially bound to loci that were already tr  ...[more]

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