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Transcription profiling of chicken bursal cells treated with stem cell antigen 2

ABSTRACT: The differentiation and proliferation of chicken B cells rely on the antigens on the cell surface of bursal stromal cells. Chicken stem cell antigen 2 (SCA2) is localized on the surface of bursal cortical and medullary epithelial cells. The signals through SCA2 may regulate the expressions critical for the B cell development. The altered expressions of bursal cells that were induced through SCA2-signaling were detailed via Affemetrix array experiments. Experiment Overall Design: Bursa of 3 week old line 0 chickens were collected and manually teased to harvest the bursal cells. The bursal cells were spread on the monolayers of SCA2Flag-DF1 cells or control Vector-DF1 cells in triplicate. After growth in the mixture of Liebovitzâs L-15 and McCoy 5A media (1:1) supplemented with 4% FBS, penicillin, and streptomycin for overnight at 37 ÂºC in 5% CO2, the bursal cells were recovered by washes (DF1 cells are adhesive) followed by gentle centrifugation at 1000Ãg for 10 min. Total RNA was isolated using the absolutely RNA microprep kit.

ORGANISM(S): Gallus gallus

SUBMITTER: Weifeng Mao

PROVIDER: E-GEOD-18506 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from chicken bursal cells treated with stem cell antigen 2

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