Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Drosophila DHR96 nuclear receptor mutants

ABSTRACT: DHR96 is a Drosophila member of the CAR/PXR/LXR family of Nuclear receptors. To Identify potential target genes for the DHR96 nuclear receptor we used microarray analysis to identify genes that were mis-regulated relative to control animals in the DHR96 mutant. Experiment Overall Design: Total RNA was isolated from mature adult DHR96 mutant and genetically matched Canton S controls and subjected to affymetrix microarray analysis.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Carl Thummel

PROVIDER: E-GEOD-18576 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The DHR96 nuclear receptor controls triacylglycerol homeostasis in Drosophila.

Sieber Matthew H MH Thummel Carl S CS

Cell metabolism 20091201 6

Triacylglycerol (TAG) homeostasis is an integral part of normal physiology and essential for proper energy metabolism. Here we show that the single Drosophila ortholog of the PXR and CAR nuclear receptors, DHR96, plays an essential role in TAG homeostasis. DHR96 mutants are sensitive to starvation, have reduced levels of TAG in the fat body and midgut, and are resistant to diet-induced obesity, while DHR96 overexpression leads to starvation resistance and increased TAG levels. We show that DHR96 ...[more]

PMID: 19945405

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Project description:To investigate the systemic molecular changes occurring as a result of Dhr96 knockdown or over-expression, a comparison between knockdown or overexpression lines and their genetic controls were performed. 0-3 day old adult males or females were reared on 3 separate batches of diet (this was the standard diet we used for culturing Drosophila melanogaster and was made up of 10L water, 100g agar (USP #7060 Bio-serve), 350g Brewer’s dried yeast (Sunshine Health), 300g black treacle (Lyles), 150g sucrose (Tate & Lyle), 300g Difco dextrose (Becton Dickinson), 150g cornmeal (#1151, Bioserve), 100g wheatgerm (#1659, Bioserve), 200g soya bean flour (#S9633 Sigma Aldrich), 10g methyl-4-hydroxybenzoate (#H3647 Sigma Aldrich) in 10ml ethanol, 50ml proprionic acid (#P5561 Sigma Aldrich)). Each of these 3 batches was considered to represent independent biological replication. The RNA samples were hybridized to the Affymetrix Drosophila GeneChip 2. Previous Q-PCR analysis had indicated that there was differential Dhr96 gene expression in the knockdown or overexpression strains compared to their controls. (This was also inferred from phenotypes resulting from other distinct crosses.) However the first analysis carried out on these samples was to verify the levels of differential Dhr96 gene expression. From this analysis we found no evidence that Dhr96 is down-regulated in the knockdown line compared to its control for either Males or Females, as the confidence intervals for the fold change include the value of one and the p-values are large. Therefore the knockdown line and its control samples were excluded from further analysis. The overexpression line did however show significant up-regulation of Dhr96 expression relative to its control in both Males and Females, and was further analyzed. Flies of the appropriate genotype (Dhr96 knockdown or over-expression and their genetic controls) were reared on 3 different batches of diet (same recipe, prepared on different days). 0-3 day old adults were collected (males and females stored seperately) and each sample was hybridised independently to a chip.

Dataset Information

Transcription profiling of Drosophila DHR96 nuclear receptor mutants

Publications

The DHR96 nuclear receptor controls triacylglycerol homeostasis in Drosophila.

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