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Comparative Genomic Hybridizations of Mycoplasma gallisepticum Vaccine strains and strain Rlow

ABSTRACT: Genomic content of Vaccine strains were probed against the known sequence of the virulent strain Rlow of M. gallisepticum to identify divergent or absent genes in the attenuated strains. Genomic DNA was extracted from in vitro grown cultures of M. gallisepticum strains Rlow, F, ts-11, and 6/85 and three samples from each were selected for hybirdization to oligonucleotide microarrays using standard methods from the Bioprime CGH kit. All samples were labeled with Cy3 dye and scanned at a PMT gain of 700 using a GenePix 4000B scanner. Features are duplicated on the slides and data were averaged between duplicate features on each slide. Median signal intensities were averaged between samples of the same strain and each feature from each vaccine strain was compared to Rlow. Features exhibiting four-fold or less hybridization in the vaccine strains were considered divergent or absent.

ORGANISM(S): Mycoplasma gallisepticum

SUBMITTER: T Gorton 

PROVIDER: E-GEOD-19629 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparative genomic analyses of attenuated strains of Mycoplasma gallisepticum.

Szczepanek S M SM   Tulman E R ER   Gorton T S TS   Liao X X   Lu Z Z   Zinski J J   Aziz F F   Frasca S S   Kutish G F GF   Geary S J SJ  

Infection and immunity 20100201 4

Mycoplasma gallisepticum is a significant respiratory and reproductive pathogen of domestic poultry. While the complete genomic sequence of the virulent, low-passage M. gallisepticum strain R (R(low)) has been reported, genomic determinants responsible for differences in virulence and host range remain to be completely identified. Here, we utilize genome sequencing and microarray-based comparative genomic data to identify these genomic determinants of virulence and to elucidate genomic variabili  ...[more]

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