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Bifidobacterium bifidum BF-1 Suppresses Helicobacter pylori-Induced genes in Human Epithelial Cells

ABSTRACT: We have shown that pre-incubation with Bifidobacterium bifidum (B. bifidum) strain BF-1, a probiotic strain known to improve H. pylori-associated gastritis, suppresses the induction of IL-8 by the pathogen. To investigate how BF-1 affects gene expression in H. pylori-infected cells, we performed microarray analysis to assess gene expression in epithelial cells, which had been pre-incubated with BF-1 and infected with H. pylori. Four-condition experiment. Biological replicates with a dye-exchange experiment. Biological replicates with a longer incubation time experiment.

ORGANISM(S): Homo sapiens

SUBMITTER: Harue Shibahara-Sone

PROVIDER: E-GEOD-20171 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Bifidobacterium bifidum BF-1 suppresses Helicobacter pylori-induced genes in human epithelial cells.

Shirasawa Y Y Shibahara-Sone H H Iino T T Ishikawa F F

Journal of dairy science 20101001 10

Helicobacter pylori infection alters gene expression in host cells. Specifically, inflammatory chemokines such as IL-8 are upregulated in the gastric mucosa during H. pylori infection. Although the mechanism by which H. pylori causes inflammation of the gastric mucosa is not yet understood, many studies have suggested that nuclear factor kappa B (NF-κB) plays a key regulatory role in host cells. We have shown that preincubation with Bifidobacterium bifidum strain BF-1, a probiotic strain known t ...[more]

PMID: 20854986

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