Unknown,Transcriptomics,Genomics,Proteomics

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Effect of BMP4 and noggin on gene expression in murine R1 ES cells


ABSTRACT: To confirm that the SMAD1/5- and SMAD4-associated genes are direct transcriptional regulators in mESCs in response to BMP, we treated undifferentiated R1 ES cells with BMP4 or with the BMP4 antagonist noggin, which can inhibit BMP signaling effectively for 4 h. Undifferentiated R1 ES cells were treated for 4 h with BMP4 or with the BMP4 antagonist noggin, which can inhibit BMP signaling effectively. Untreated R1 ES cells served as the control.

ORGANISM(S): Mus musculus

SUBMITTER: Zhang Chen 

PROVIDER: E-GEOD-20527 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide mapping of SMAD target genes reveals the role of BMP signaling in embryonic stem cell fate determination.

Fei Teng T   Xia Kai K   Li Zhongwei Z   Zhou Bing B   Zhu Shanshan S   Chen Hua H   Zhang Jianping J   Chen Zhang Z   Xiao Huasheng H   Han Jing-Dong J JD   Chen Ye-Guang YG  

Genome research 20091119 1


Embryonic stem (ES) cells are under precise control of both intrinsic self-renewal gene regulatory network and extrinsic growth factor-triggered signaling cascades. How external signaling pathways connect to core self-renewal transcriptional circuits is largely unknown. To probe this, we chose BMP signaling, which is previously recognized as a master control for both self-renewal and lineage commitment of murine ES cells. Here, we mapped target gene promoter occupancy of SMAD1/5 and SMAD4 on a g  ...[more]

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